Lu Yu, Chai Weizhong, Lin Xiaoming
Department of Food and Nutritional Health, College of Public Health, Beijing University Medical School, Beijing.
Wei Sheng Yan Jiu. 2007 Jan;36(1):37-40.
To investigate the effect of milk basic protein (MBP)'s on bone mineral density (BMD) and hemobiochemical indexes in normal and ovariectomized (ovx) rats.
48 female Sprague-Dawley rats were ovariectomized and another 12 rats received a sham operation (sham). The ovx rats were randomly separated into 4 groups: ovx control, ovx low-does, ovx media dose, and ovx high-dose while 44 normal SD female rats into 4 groups: normal control, normal low-dose, normal media-dose, and normal high-dose. MBP at 10, 20 and 30 mg/(kg x d) were applied in rat diet for continuous 90 days. BMD (at proximal, middle of diaphysis, and distal end) and bone mineral content (BMC) was measured by dual energy X-ray absorptiomery. In addition, serum total protein (TP), total cholesterol (CHO), glucose (GLU) were determined.
The bone density and mineral composition at distant end of femur in low-dosage BMP group were significantly higher than those in normal control group, while no significant change of bone density was observed with MBP treatment in ovx groups. Total serum protein level and albumin level were significantly higher in MBP treatment groups than those in normal control group.
MBP at 10 mg/kg bw significantly elevated femoral distant-end bone density, as well as the total blood protein level and albumin level in normal rat. However, similar effect was not observed in ovx rat.
研究乳碱性蛋白（MBP）对正常及去卵巢（ovx）大鼠骨密度（BMD）和血液生化指标的影响。
48只雌性Sprague-Dawley大鼠接受卵巢切除术，另外12只大鼠接受假手术（假手术组）。去卵巢大鼠随机分为4组：去卵巢对照组、去卵巢低剂量组、去卵巢中剂量组和去卵巢高剂量组，而44只正常SD雌性大鼠分为4组：正常对照组、正常低剂量组、正常中剂量组和正常高剂量组。将10、20和30mg/（kg×d）的MBP添加到大鼠饮食中，持续90天。通过双能X线吸收法测量骨密度（股骨近端、骨干中部和远端）和骨矿物质含量（BMC）。此外，测定血清总蛋白（TP）、总胆固醇（CHO）、葡萄糖（GLU）。
低剂量BMP组股骨远端的骨密度和矿物质组成显著高于正常对照组，而去卵巢组MBP处理后骨密度未观察到显著变化。MBP处理组的血清总蛋白水平和白蛋白水平显著高于正常对照组。
10mg/kg体重的MBP显著提高了正常大鼠股骨远端的骨密度以及全血蛋白水平和白蛋白水平。然而，在去卵巢大鼠中未观察到类似效果。
