Wang Xiaoying, Liu Xiumei
Institute of Nutrition and Food safety, Chinese Center for Disease Control and Food Safety, Beijing 100050, China.
Wei Sheng Yan Jiu. 2007 Jan;36(1):55-9.
To study the phylogenetic relationships of fumonisin-producing Fusarium verticillioides isolates from China and Gibberella fujikuroi species complex.
5.8S, 28S rDNA and ITS1 and ITS2 sequences of 4 ATCC typical strains and 12 isolates from different geographical regions and different samples in China and other countries were determined and analyzed. The results were submitted to GenBank. Phylogenetic tree and homology tree were illustrated. The characteristics of etiological distribution of toxigenetic Fusarium verticillioides and the relativity of of rDNA ITS2 sequence and the fumS gene was elucidated by comparing the isolates from China, South Africa and USA.
It was found that rDNA highly devergent ITS2 sequences of our isolates with positive fumonisin biosynthase gene were type I, which was consistant with fumonisin-producing isolates from South Africa. The Fusarium verticillioides isolates with ITS2 type II sequence were fumS gene negative. Two Fusarium subglutinans isolates SD-fmv047 and SD-fmv049 were nonfumonisin-producing and ITS2 sequences were neither type I nor II. The DNA sequences had high homology with Fusarium oxysporum.
New and reliable theory and technology supports were provided for the further study of phylogenetic relationships of Gibberella fujikuroi species complex.
研究来自中国的产伏马毒素的轮枝镰孢菌分离株与藤仓赤霉菌复合种之间的系统发育关系。
测定并分析了4株ATCC典型菌株以及来自中国和其他国家不同地理区域和不同样品的12株分离株的5.8S、28S核糖体DNA（rDNA）以及内转录间隔区1（ITS1）和内转录间隔区2（ITS2）序列。结果提交至GenBank。绘制了系统发育树和同源性树。通过比较来自中国、南非和美国的分离株，阐明了产毒轮枝镰孢菌的病因分布特征以及rDNA ITS2序列与伏马毒素合成酶基因（fumS基因）的相关性。
发现我们分离得到的具有伏马毒素生物合成酶基因阳性的分离株的rDNA ITS2序列高度分化，为I型，这与来自南非的产伏马毒素分离株一致。具有ITS2 II型序列的轮枝镰孢菌分离株fumS基因呈阴性。两株亚粘团镰孢菌分离株SD-fmv047和SD-fmv049不产伏马毒素，其ITS2序列既不是I型也不是II型。这些DNA序列与尖孢镰孢菌具有高度同源性。
为进一步研究藤仓赤霉菌复合种的系统发育关系提供了新的可靠的理论和技术支持。
