Kirsenko O V, Chupyra V V
Ukr Biokhim Zh. 1975 Mar-Apr;47(2):139-45.
Mg2+, Na+, K+-ATPase and K+-acetylphosphatase activities in the brain subcellular membrane structures were studied as affected by ultrasound. Effect of ultrasound depends both on the conditions of sonication and the initial state of membrane fractions. The ATPase activity in the NaI-treated microsomal fraction decreases after sonication. The treatment of microsomal myelin, synaptosomal and mitochondrial fractions with EDTA solutions (5-10(-5)--2-10(-2) M) causes an inhibition in the Mg2+, Na+, K+-ATPase. The ATPase activity of these so-called EDTA-fractions increases after a short-term sonication. Activation of the Mg2+, Na+, K+-ATPase of EDTA-microsomal fraction with a sonication is accompanied by an activation of K+-acetylphosphatase. In contrast to the ATPase activity, the acetylphosphatase one is manifested after a longer effect of ultrasound as well. The maximum of acetylphosphatase activation with sonication coincides with the maximum of Mg2+, Na+, K+-ATPase activation. The content of unmasked SH-groups in the studied fractions decreases after sonication. When EDTA-fractions are affected by sonication, which is accompanied by an increase in the ATPase activity, the number of SH-groups in all the fractions, except for mitochondria, increases.
研究了超声对脑亚细胞膜结构中Mg2+、Na+、K+-ATP酶和K+-乙酰磷酸酶活性的影响。超声的作用既取决于超声处理条件，也取决于膜组分的初始状态。超声处理后，用NaI处理的微粒体组分中的ATP酶活性降低。用EDTA溶液（5×10-5 - 2×10-2 M）处理微粒体髓磷脂、突触体和线粒体组分，会抑制Mg2+、Na+、K+-ATP酶。这些所谓的EDTA组分在短期超声处理后，其ATP酶活性增加。超声处理激活EDTA-微粒体组分的Mg2+、Na+、K+-ATP酶的同时，也伴随着K+-乙酰磷酸酶的激活。与ATP酶活性不同，乙酰磷酸酶活性在超声作用较长时间后也会表现出来。超声处理使乙酰磷酸酶激活的最大值与Mg2+、Na+、K+-ATP酶激活的最大值一致。超声处理后，所研究组分中暴露的SH基团含量降低。当EDTA组分受到超声处理且伴随着ATP酶活性增加时，除线粒体之外的所有组分中的SH基团数量都会增加。
