Lierman S, De Sutter P, Liu J, Gerris J, Dhont M, Van der Elst J
Infertility Centre, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium.
Reprod Biomed Online. 2007 Apr;14(4):436-43. doi: 10.1016/s1472-6483(10)60890-5.
In-vitro culture of ovarian follicles offers the possibility of checking whether there are direct interactions between follicles. Gross follicle morphology and oocyte maturation were investigated as endpoints after co-culture of pre-antral mouse ovarian follicles. Pre-antral mouse follicles with a diameter of 95-110 microm (small) or >110-160 microm (large) were cultured. Pairs of different size or large like-sized follicles were cultured either in physical contact or without in 20 microl culture medium droplets under oil for 10 days. Individual culture of small and large follicles served as controls. On day 10 human chorionic gonadotrophin was administered. On day 11, 'in-vitro ovulated' cumulus-oocyte complexes where the cumuli oophori had expanded (termed 'mucified') were collected. The percentage of metaphase II (MII) oocytes was scored as primary endpoint. There were temporary differences between the degree of follicle attachment and diffusion of granulosa cells, but with the observations of antrum formation by day 10, there was no difference between different types of co-cultures and individual culture of large follicles. The MII-to-follicle rate was highest for individual culture of large follicles (54.7%) followed by coculture of large like-sized follicles growing either in contact or not (40.1% and 42.6%), co-culture of different-sized follicles growing in contact or not (28.5% and 29.8%) and small follicles cultured individually (8.1%). In conclusion, mature oocyte yield was not increased by co-culture of mouse pre-antral ovarian follicles.
卵巢卵泡的体外培养为检查卵泡之间是否存在直接相互作用提供了可能性。将小鼠窦前卵巢卵泡共培养后，研究了卵泡的总体形态和卵母细胞成熟情况作为终点指标。培养直径为95 - 110微米（小）或>110 - 160微米（大）的小鼠窦前卵泡。将不同大小或大小相似的卵泡对在有物理接触或无物理接触的情况下，于20微升培养基液滴中在油下培养10天。小卵泡和大卵泡的单独培养作为对照。在第10天给予人绒毛膜促性腺激素。在第11天，收集“体外排卵”的卵丘 - 卵母细胞复合体，其中卵丘已扩张（称为“黏液化”）。将中期II（MII）卵母细胞的百分比作为主要终点指标进行评分。卵泡附着程度和颗粒细胞扩散程度存在暂时差异，但到第10天观察到有腔形成时，不同类型的共培养与大卵泡单独培养之间没有差异。大卵泡单独培养的MII与卵泡比率最高（54.7%），其次是大小相似的大卵泡有接触或无接触生长的共培养（40.1%和42.6%）、不同大小卵泡有接触或无接触生长的共培养（28.5%和29.8%）以及小卵泡单独培养（8.1%）。总之，小鼠窦前卵巢卵泡共培养并未提高成熟卵母细胞的产量。
