Hashimoto Shu, Fukuda Aisaku, Murata Yasutaka, Kikkawa Miho, Oku Hirotsugu, Kanaya Hiroyuki, Sonoda Momoyo, Sugihara Kengo, Murata Tomoko, Nagata Fumie, Nakaoaka Yoshiharu, Morimoto Yoshiharu
IVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015 Japan.
Reprod Biomed Online. 2007 Apr;14(4):444-9. doi: 10.1016/s1472-6483(10)60891-7.
In-vitro maturation (IVM) of immature oocytes has been proposed as a potential alternative to conventional IVF treatment following ovarian stimulation. However, the effects of the oocyte retrieval conditions on subsequent development have not been well understood. This study assessed the effects of different aspiration vacuums during oocyte retrieval on the developmental competence of immature oocytes following IVM, IVF and embryo transfer, retrospectively. Immature oocytes were aspirated with 20-gauge needles with a vacuum of 180 or 300 mmHg. Immature oocytes were cultured in IVM medium for 26 h. All mature oocytes were inseminated by intracytoplasmic sperm injection (ICSI). Embryo transfer was carried out 2 or 3 days after ICSI. The percentage of cumulus-cell enclosed oocytes and of transferable embryos per retrieved oocytes in 180 mmHg (69.7% and 23.8%, respectively) were significantly higher (P < 0.01) than those in 300 mmHg (46.2% and 12.8%, respectively). The ongoing pregnancy rate per retrieval cycle in 180 mmHg (30%) was higher (P < 0.01) than that in 300 mmHg (4.3%). The data indicate that lower pressure of vacuum aspiration with a 20-gauge needle improves the developmental competence of immature oocytes following IVM, IVF and embryo transfer.
未成熟卵母细胞的体外成熟（IVM）已被提议作为卵巢刺激后传统体外受精治疗的一种潜在替代方法。然而，卵母细胞采集条件对后续发育的影响尚未得到充分了解。本研究回顾性评估了卵母细胞采集过程中不同抽吸负压对未成熟卵母细胞经IVM、IVF和胚胎移植后的发育能力的影响。使用20号针头以180或300 mmHg的负压抽吸未成熟卵母细胞。将未成熟卵母细胞在IVM培养基中培养26小时。所有成熟卵母细胞均通过卵胞浆内单精子注射（ICSI）进行授精。在ICSI后2或3天进行胚胎移植。180 mmHg组中每个采集卵母细胞的卵丘细胞包裹卵母细胞百分比和可移植胚胎百分比（分别为69.7%和23.8%）显著高于300 mmHg组（分别为46.2%和12.8%）（P<0.01）。180 mmHg组每个采集周期的持续妊娠率（30%）高于300 mmHg组（4.3%）（P<0.01）。数据表明，使用20号针头进行较低负压的抽吸可提高未成熟卵母细胞经IVM、IVF和胚胎移植后的发育能力。
