Rodriguez Nancy A, Si Wei, Emmi Adelina M, Layman Lawrence C, Eroglu Ali
Laboratory Animal Services, Medical College of Georgia, CB-2803 Augusta, GA 30912, USA.
Mol Reprod Dev. 2009 Sep;76(9):890-6. doi: 10.1002/mrd.21060.
Oocytes of nonhuman primates such as rhesus monkeys are excellent models for diverse studies on developmental biology, epigenetics, human reproduction, and assisted reproductive technologies, as well as on transgenics. Such studies require numerous oocytes that can be retrieved after controlled ovarian stimulation. Currently, most primate centers use laparoscopic aspiration or laparotomy followed by aspiration to collect rhesus oocytes, although the ultrasound-guided needle aspiration is more advantageous due to reduced infection risk, less injury, and a shorter recovery period. Yet, some initial difficulties associated with the ultrasound-guided needle aspiration limit its broader application. The objective of the present study was to address these obstacles. By presenting practical solutions to the initial difficulties, results from our study show that it is possible to collect a mean number of 38 +/- 10 rhesus oocytes per hormonally stimulated female. These results compare favorably to the average number of rhesus oocytes collected using the laparoscopic approach and suggest that when initial obstacles are overcome, the ultrasound-guided oocyte retrieval represents a good alternative to more invasive approaches.
恒河猴等非人灵长类动物的卵母细胞是发育生物学、表观遗传学、人类生殖、辅助生殖技术以及转基因等多种研究的优秀模型。此类研究需要大量可在控制性卵巢刺激后获取的卵母细胞。目前，大多数灵长类动物研究中心采用腹腔镜抽吸或剖腹手术加抽吸的方法来采集恒河猴卵母细胞，尽管超声引导下经皮穿刺抽吸因感染风险降低、损伤较小且恢复期较短而更具优势。然而，与超声引导下经皮穿刺抽吸相关的一些初始困难限制了其更广泛的应用。本研究的目的是解决这些障碍。通过提出针对初始困难的实际解决方案，我们的研究结果表明，每只接受激素刺激的雌性恒河猴平均可采集到38±10枚卵母细胞。这些结果与采用腹腔镜方法采集的恒河猴卵母细胞平均数量相比更具优势，表明当克服初始障碍时，超声引导下卵母细胞采集是一种比侵入性更强的方法更好的选择。
