Wang X F, Ma J, Wang W S, Zheng Y M, Zhang G Y, Liu C J, Ma Z Y
Key Laboratory of Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding 071001, China.
Genome. 2006 Nov;49(11):1393-8. doi: 10.1139/g06-113.
As the second most widely cultivated cotton, Gossypium barbadense is well known for its superior fiber properties and its high levels of resistance to Fusarium and Verticillium wilts. To enhance our ability to exploit these properties in breeding programs, we constructed the first bacterial artificial chromosome (BAC) library for this species. The library contains 167 424 clones (49 920 BamHI and 117 504 HindIII clones), with an estimated average insert size of 130 kb. About 94.0% of the clones had inserts over 100 kb, and the empty clones accounted for less than 4.0%. Contamination of the library with chloroplast clones was very low (0.2%). Screening the library with locus-specific probes showed that BAC clones represent 6.5-fold genome equivalents. This high-quality library provides an additional asset with which to exploit genetic variation for cotton improvement.
作为第二大广泛种植的棉花，海岛棉以其优良的纤维特性以及对枯萎病和黄萎病的高抗性而闻名。为了提高我们在育种计划中利用这些特性的能力，我们构建了该物种的第一个细菌人工染色体（BAC）文库。该文库包含167424个克隆（49920个BamHI克隆和117504个HindIII克隆），估计平均插入片段大小为130 kb。约94.0%的克隆插入片段超过100 kb，空克隆占比不到4.0%。文库中叶绿体克隆的污染率非常低（0.2%）。用位点特异性探针筛选文库表明，BAC克隆代表6.5倍的基因组当量。这个高质量的文库为利用棉花改良的遗传变异提供了又一宝贵资源。
