North China Key Laboratory for Germplasm Resources of Education Ministry, Department of Plant Genetics and Breeding, Agricultural University of Hebei, Baoding, 071001, People's Republic of China.
Plant Cell Rep. 2011 Nov;30(11):2085-96. doi: 10.1007/s00299-011-1115-x. Epub 2011 Jul 8.
Verticillium wilt causes enormous loss to yield or quality in many crops. In an effort to help controlling this disease through genetic engineering, we first cloned and characterized a Verticillium wilt resistance gene (GbVe) from cotton (Gossypium barbadense) and analyzed its function in Arabidopsis thaliana. Its nucleotide sequence is 3,819 bp long, with an open reading frame of 3,387 bp, and encoding an 1,128-aa protein precursor. Sequence analysis shows that GbVe produces a leucine-rich repeat receptor-like protein. It shares identities of 55.9% and 57.4% with tomato Ve1 and Ve2, respectively. Quantitative real-time PCR indicated that the Ve gene expression pattern was different between the resistant and susceptible cultivars. In the resistant Pima90-53, GbVe was quickly induced and reached to a peak at 2 h after inoculation, two-fold higher than that of control. We localized the GbVe-GFP fusion protein to the cytomembrane in onion epidermal cells. By inserting GbVe into Arabidopsis via Agrobacterium-mediated transformation, T(3) transgenic lines were obtained. Compared with the wild-type control, GbVe-overexpressing plants had greater levels of resistance to V. dahliae. This suggests that GbVe is a useful gene for improving the plant resistance against fungal diseases.
黄萎病会给许多作物的产量和品质造成巨大损失。为了通过基因工程帮助控制这种疾病，我们首先从棉花（Gossypium barbadense）中克隆并鉴定了一个黄萎病抗性基因（GbVe），并分析了其在拟南芥中的功能。其核苷酸序列长 3819bp，开放阅读框长 3387bp，编码一个 1128 个氨基酸的蛋白前体。序列分析表明，GbVe 产生一个富含亮氨酸重复的受体样蛋白。它与番茄 Ve1 和 Ve2 的同源性分别为 55.9%和 57.4%。实时定量 PCR 表明，抗性和感病品种的 Ve 基因表达模式不同。在抗性品种 Pima90-53 中，GbVe 迅速诱导，接种后 2 小时达到峰值，是对照的两倍。我们将 GbVe-GFP 融合蛋白定位到洋葱表皮细胞的细胞质膜上。通过将 GbVe 插入拟南芥中，通过农杆菌介导的转化获得了 T(3)转基因株系。与野生型对照相比，GbVe 过表达植株对黄萎病菌的抗性更强。这表明 GbVe 是提高植物对真菌病害抗性的有用基因。
