Muscat Sonja, Pelka Joana, Hegele Jörg, Weigle Bernd, Münch Gerald, Pischetsrieder Monika
Institute of Pharmacy and Food Chemistry, Friedrich-Alexander-University, Erlangen, Germany.
Mol Nutr Food Res. 2007 May;51(5):525-35. doi: 10.1002/mnfr.200600254.
In this study, we investigated the immunomodulatory activity of coffee and Maillard reaction products on macrophages in vitro. Stimulation of macrophages with coffee, but not with raw coffee extract in PBS, led to a 13-fold increased nuclear NF-kappaB translocation. A Maillard reaction mixture (25 mM D-ribose/L-lysine, 30 min at 120 degrees C) increased NF-kappaB translocation 18-fold (in PBS) or six-fold (in medium). MRPs also induced a two-fold increased NF-kappaB translocation in untransfected human embryonic kidney (HEK) cells as well as in HEK cells stably transfected with the receptor for advanced glycation endproducts (RAGE), indicating that the effect was not RAGE mediated. On the other hand, catalase totally abolished coffee- and MRP-induced NF-kappaB translocation. Consequently, up to 366 microM hydrogen peroxide was measured in the coffee preparation and Maillard mixtures used for cell stimulation. Stimulation of macrophages with MRPs did not lead to significantly increased IL-6 or NO release. Thus, it can be concluded that coffee and MRPs induce NF-kappaB translocation in macrophages via the generation of hydrogen peroxide.
在本研究中，我们体外研究了咖啡和美拉德反应产物对巨噬细胞的免疫调节活性。用咖啡刺激巨噬细胞，但不用PBS中的生咖啡提取物刺激，导致核NF-κB易位增加13倍。美拉德反应混合物（25 mM D-核糖/L-赖氨酸，120℃ 30分钟）使NF-κB易位增加18倍（在PBS中）或6倍（在培养基中）。美拉德反应产物还使未转染的人胚肾（HEK）细胞以及稳定转染晚期糖基化终产物受体（RAGE）的HEK细胞中的NF-κB易位增加两倍，表明该效应不是由RAGE介导的。另一方面，过氧化氢酶完全消除了咖啡和美拉德反应产物诱导的NF-κB易位。因此，在用于细胞刺激的咖啡制剂和美拉德混合物中测得高达366 microM的过氧化氢。用美拉德反应产物刺激巨噬细胞不会导致IL-6或NO释放显著增加。因此，可以得出结论，咖啡和美拉德反应产物通过过氧化氢的产生诱导巨噬细胞中的NF-κB易位。
