Wang Shaohua, Li Jing, Xia Wei, Geng Meiyu
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China.
Neurol Res. 2007 Jan;29(1):96-102. doi: 10.1179/174313206X152483.
In Alzheimer's disease (AD), beta-amyloid (Abeta) plaques are surrounded by activated astrocytes and microglia. A growing body of evidence suggests that these activated astrocytes contribute to neurotoxicity through the induction of inflammatory cytokines and the production of oxidative stress mediators. Thus, a compound inhibiting Abeta-induced activation of astrocytes may lead to a novel therapy for AD. Our current work investigates the roles of acidic oligosaccharide sugar chain (AOSC), derived from brown algae Echlonia Kurome Okam, on Abeta-induced inflammatory responses and cytotoxicity. We observed that AOSC inhibited the toxicity and apoptosis in SH-SY5Y cell line induced by Abeta-stimulated astrocytes conditioned medium. We found that AOSC inhibited the reactive phenotype of astrocytes, blocked cellular oxidative stress, reduced the production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 and prevented the influx of Ca2+. Thus, our results indicate that AOSC might be a potentially therapeutic compound for AD.
在阿尔茨海默病（AD）中，β-淀粉样蛋白（Aβ）斑块被活化的星形胶质细胞和小胶质细胞所包围。越来越多的证据表明，这些活化的星形胶质细胞通过诱导炎性细胞因子和产生氧化应激介质而导致神经毒性。因此，一种抑制Aβ诱导的星形胶质细胞活化的化合物可能会成为治疗AD的新方法。我们目前的工作研究了源自褐藻昆布（Echlonia Kurome Okam）的酸性寡糖糖链（AOSC）对Aβ诱导的炎症反应和细胞毒性的作用。我们观察到AOSC抑制了由Aβ刺激的星形胶质细胞条件培养基诱导的SH-SY5Y细胞系中的毒性和凋亡。我们发现AOSC抑制了星形胶质细胞的反应性表型，阻断了细胞氧化应激，减少了肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的产生，并阻止了Ca2+的内流。因此，我们的结果表明AOSC可能是一种潜在的AD治疗化合物。
