Xiao Fang-Yi, Zhang Huai-Qin, Yu Hua, Yang De-Ye, Huang Wei-Jian, Zhou Hao
Department of Cardiology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Apr;23(4):303-5.
To investigate how to isolate, culture and identify two types of endothelial progenitor cells from peripheral blood in rabbits.
Mononuclear cells(MNCs) were isolated from rabbit peripheral blood. Endothelial progenitor cells (EPCs) and endothelial outgrowth cells (EOCs) were obtained from MNCs through different ways of isolation and culture. Two types of cells were assessed by DiI-ac-LDL uptake and lectin binding, and then they were identified by immunofluorescence of flk-1, immunocytochemistry of CD34 and VIII factor related antigen and vasculogenesis activity in vitro.
Two types of endothelial progenitor cells were obtained from rabbit peripheral blood through different ways of isolation and culture. EPCs on the seventh day and EOCs on the sixteenth day were positive for ac-LDL uptake and lectin binding, and both of them expressed CD34, flk-1 and VIII factor related antigen. EOCs were assembled into primitive vascular tube-like structures when plated in matrigel.
EPCs and EOCs could be obtained from rabbit peripheral blood when different ways of isolation and culture were performed. The system of cell culture can be applied to subsequent experiments in cell transplantation.
探讨如何从兔外周血中分离、培养和鉴定两种内皮祖细胞。
从兔外周血中分离单个核细胞（MNCs）。通过不同的分离和培养方法从MNCs中获得内皮祖细胞（EPCs）和内皮游走细胞（EOCs）。通过DiI-乙酰化低密度脂蛋白摄取和凝集素结合对两种细胞进行评估，然后通过flk-1免疫荧光、CD34免疫细胞化学和VIII因子相关抗原以及体外血管生成活性对其进行鉴定。
通过不同的分离和培养方法从兔外周血中获得了两种内皮祖细胞。第7天的EPCs和第16天的EOCs对乙酰化低密度脂蛋白摄取和凝集素结合呈阳性，且二者均表达CD34、flk-1和VIII因子相关抗原。EOCs接种于基质胶中时可组装成原始血管管状结构。
采用不同的分离和培养方法可从兔外周血中获得EPCs和EOCs。该细胞培养体系可应用于后续的细胞移植实验。
