Chen Li-Na, Wang Cong-Hua, Zhu Ping, Fan Chun-Mei, Wang Yan-Hong, Li Xiao-Yan
Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Apr;23(4):331-4.
To explore the percentages of CD4(+) CD25(high) regulatory T cells in peripheral blood or synovial fluid from patients with rheumatoid arthritis (RA) on different stages, and to study their correlations with the activity of the disease.
Peripheral blood lymphocytes were obtained from the patients with active RA who had received no previous disease modifying (DMARDs) therapy(n=11), from individuals with stable, well-controlled RA (n=12), from subjects whose disease were poorly improved after DMARDs therapy(n=9), and from healthy controls(n=8). The frequencies of CD4(+) CD25(high) T cells were quantified by using flow cytometry(FCM). Meanwhile, the correlations between the percentage of CD4(+) CD25(high) T cells and the level of Anti-CCP antibody, CRP, ESR and RF were also investigated. Paired blood and synovial fluid was analysed in a small group of RA and other patients.
There was a smaller proportion of CD4(+) CD25(high) T cells in the peripheral blood of the active RA patients(mean 5.24%) and poorly-improved RA patients(mean 6.43%) than in patients with stable well-controlled RA or in healthy controls(mean 11.79% and 17.17%, respectively, P<0.01 in each case). The frequency of CD4(+) CD25(high) T cells from RA was negatively associated with Anti-CCP antibody(58.0 Ru/mL), ESR(38.8 mm/h) and CRP(2.73 mug/L), (P<0.05 for each). On contrast, The frequency of CD4(+) CD25(high) T cells from healthy controls was not significantly correlated with the level of Anti-CCP antibody(<5.0 Ru/mL), ESR(4.67 mm/h), CRP(0.15 mug/L) and RF(1.37) (all P>0.1). The percentage of CD4(+) CD25(high) regulatory T cells from synovial fluid of RA patients (24.32%) was significantly lower than that of AS patients(30.24%) (P<0.05).
The results demonstrate a smaller CD4(+) CD25(high) regulatory T cell population in peripheral blood of individuals with active RA prior to disease modifying treatment and with poorly-improved RA, and this is negatively associated with the activity of the disease.
探讨不同病程类风湿关节炎（RA）患者外周血及滑膜液中CD4(+)CD25(high)调节性T细胞的比例，并研究其与疾病活动度的相关性。
采集未接受过改善病情抗风湿药（DMARDs）治疗的活动期RA患者（n = 11）、病情稳定且控制良好的RA患者（n = 12）、DMARDs治疗后病情改善不佳的患者（n = 9）及健康对照者（n = 8）的外周血淋巴细胞。采用流式细胞术（FCM）定量检测CD4(+)CD25(high) T细胞频率。同时，研究CD4(+)CD25(high) T细胞百分比与抗环瓜氨酸肽（Anti-CCP）抗体、C反应蛋白（CRP）、红细胞沉降率（ESR）及类风湿因子（RF）水平的相关性。对一小部分RA患者及其他患者的配对血液和滑膜液进行分析。
活动期RA患者外周血（平均5.24%）及病情改善不佳的RA患者外周血（平均6.43%）中CD4(+)CD25(high) T细胞比例低于病情稳定且控制良好的RA患者或健康对照者（分别为平均11.79%和17.17%，每组P<0.01）。RA患者CD4(+)CD25(high) T细胞频率与抗CCP抗体（58.0 Ru/mL）、ESR（38.8 mm/h）及CRP（2.73 μg/L）呈负相关（每组P<0.05）。相比之下，健康对照者CD4(+)CD25(high) T细胞频率与抗CCP抗体（<5.0 Ru/mL）、ESR（4.67 mm/h）、CRP（0.15 μg/L）及RF（1.37）无显著相关性（均P>0.1）。RA患者滑膜液中CD4(+)CD25(high)调节性T细胞百分比（24.32%）显著低于强直性脊柱炎（AS）患者（30.24%）（P<0.05）。
结果表明，在接受病情改善治疗前的活动期RA患者及病情改善不佳的RA患者外周血中，CD4(+)CD25(high)调节性T细胞数量较少，且与疾病活动度呈负相关。
