Yang Yang, He Qiang, Duan Li, Cui Yue, Li Junbai
Beijing National Laboratory for Molecular Sciences (BNLMS), International Joint Lab, CAS Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Biomaterials. 2007 Jul;28(20):3083-90. doi: 10.1016/j.biomaterials.2007.03.019. Epub 2007 Mar 21.
Biodegradable nanotubes were fabricated through the layer-by-layer (LbL) assembly technique of alternate adsorption of alginate (ALG) and chitosan (CHI) onto the inner pores of polycarbonate template with the subsequent removal of the template. The assembled materials present good film formation ability. The thickness of nanotubes wall can be controlled by changing the assembled layers. The assembled tubular structure was verified by the confocal laser scanning microscope (CLSM) using fluorescent-labeled ALG as well as the measurements of scan electron microscope (SEM) and transmission electron microscopy (TEM). Atomic force microscopy (AFM) images confirm the biodegradable feature of the assembled nanotubes as they are immersed in the pancreatin. Confocal microscopy images show that the assembled ALG/CHI nanotubes can be internalized into the cancer cell readily. The cell viability experiment proves the low cytotoxicity of ALG/CHI nanotubes. The final assembled nanotubes have presented good biodegradability and low cytotoxicity.
通过将藻酸盐（ALG）和壳聚糖（CHI）交替吸附到聚碳酸酯模板的内孔上并随后去除模板的层层（LbL）组装技术制备了可生物降解的纳米管。组装后的材料具有良好的成膜能力。纳米管壁的厚度可以通过改变组装层数来控制。使用荧光标记的ALG的共聚焦激光扫描显微镜（CLSM）以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）测量验证了组装的管状结构。原子力显微镜（AFM）图像证实了组装的纳米管浸入胰蛋白酶中时的可生物降解特性。共聚焦显微镜图像显示，组装的ALG/CHI纳米管可以很容易地内化到癌细胞中。细胞活力实验证明了ALG/CHI纳米管的低细胞毒性。最终组装的纳米管具有良好的生物降解性和低细胞毒性。
