Tao Xia, Chen Hua, Sun Xue-Jun, Chen Jian-Feng, Roa Wilson H
Key Lab for Nanomaterials of the Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China.
Int J Pharm. 2007 May 24;336(2):376-81. doi: 10.1016/j.ijpharm.2006.12.009. Epub 2006 Dec 12.
A bio-polyelectrolyte microshell composed of alginate sodium (ALG) and chitosan (CHI) was fabricated by electrostatic layer-by-layer (LbL) self-assembly technique. The resulting ALG-CHI microshells were found to be able to effectively load anti-cancer drug doxorubicin (DOX) in the interior of the shells under modest conditions without addition of other reagents, as demonstrated by confocal laser scanning microscopy (CLSM). The mass of DOX loaded in one capsule of four alginate/chitosan layers (i.e. the volume V=2.5x10(-10) cm3) is calculated as ca. 1.4x10(-13) g, which corresponds to 1.5x10(8) DOX molecules. Also, the release of DOX in the shells is dependent on the number of assembled layers of the shells. Colorimetric XTT cell viability assay results showed that the DOX-loaded microshells at high concentrations tested could kill cancer cells more efficiently than free-DOX alone.
通过静电层层（LbL）自组装技术制备了一种由海藻酸钠（ALG）和壳聚糖（CHI）组成的生物聚电解质微壳。共聚焦激光扫描显微镜（CLSM）表明，在适度条件下，无需添加其他试剂，所得的ALG-CHI微壳能够在壳内部有效地负载抗癌药物阿霉素（DOX）。计算出四层海藻酸盐/壳聚糖（即体积V = 2.5x10(-10) cm3）的一个胶囊中负载的DOX质量约为1.4x10(-13) g，这相当于1.5x10(8)个DOX分子。此外，壳中DOX的释放取决于壳的组装层数。比色XTT细胞活力测定结果表明，在高浓度下测试的负载DOX的微壳比单独的游离DOX更能有效地杀死癌细胞。
