猴疱疹病毒末端重复区域的诱变揭示了病毒产生的重要元件。
Mutagenesis of the herpesvirus saimiri terminal repeat region reveals important elements for virus production.
作者信息
White Robert E, Carline Lindsay, Allday Martin J
机构信息
Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom.
出版信息
J Virol. 2007 Jun;81(12):6765-70. doi: 10.1128/JVI.02579-06. Epub 2007 Apr 11.
Abstract
Deletion of the terminal repeats (TR) from herpesvirus saimiri (HVS) renders it unable to produce infectious virus or generate plaques. However, a TR-deleted HVS bacterial artificial chromosome can form replication compartments. Complementation of this mutant shows that one copy of the TR, plus the right junction of the genome with the TR, is sufficient for efficient plaque formation and generation of infectious virus. Within the TR unit, the region around the cleavage site of the genome appears both necessary and sufficient for virus production. Analysis of episomes from productive cells indicates a propensity to amplify TR numbers during the lytic cycle.
摘要
从猴疱疹病毒（HVS）中删除末端重复序列（TR）会使其无法产生感染性病毒或形成噬斑。然而，一个删除了TR的HVS细菌人工染色体能够形成复制区室。对该突变体的互补分析表明，一份TR拷贝加上基因组与TR的右连接区，就足以高效形成噬斑并产生感染性病毒。在TR单元内，基因组切割位点周围的区域对于病毒产生似乎既是必需的也是足够的。对生产性细胞中附加体的分析表明，在裂解周期中存在扩增TR数量的倾向。
相似文献
1
Mutagenesis of the herpesvirus saimiri terminal repeat region reveals important elements for virus production.猴疱疹病毒末端重复区域的诱变揭示了病毒产生的重要元件。
J Virol. 2007 Jun;81(12):6765-70. doi: 10.1128/JVI.02579-06. Epub 2007 Apr 11.
2
Generation and precise modification of a herpesvirus saimiri bacterial artificial chromosome demonstrates that the terminal repeats are required for both virus production and episomal persistence.猿猴疱疹病毒细菌人工染色体的构建及精确修饰表明，末端重复序列对于病毒产生和游离型持续存在均是必需的。
J Gen Virol. 2003 Dec;84(Pt 12):3393-3403. doi: 10.1099/vir.0.19387-0.
3
The terminal repeats and latency-associated nuclear antigen of herpesvirus saimiri are essential for episomal persistence of the viral genome.猴疱疹病毒的末端重复序列和潜伏相关核抗原对于病毒基因组的附加体持久性至关重要。
J Gen Virol. 2002 Sep;83(Pt 9):2269-2278. doi: 10.1099/0022-1317-83-9-2269.
4
ORF73 of herpesvirus Saimiri strain C488 tethers the viral genome to metaphase chromosomes and binds to cis-acting DNA sequences in the terminal repeats.松鼠猴疱疹病毒C488株的开放阅读框73将病毒基因组与中期染色体相连，并与末端重复序列中的顺式作用DNA序列结合。
J Virol. 2003 Dec;77(23):12494-506. doi: 10.1128/jvi.77.23.12494-12506.2003.
5
The insulator protein CTCF binding sites in the orf73/LANA promoter region of herpesvirus saimiri are involved in conferring episomal stability in latently infected human T cells.猴疱疹病毒 saimiri 中 orf73/LANA 启动子区域的绝缘子蛋白 CTCF 结合位点参与赋予潜伏感染的人 T 细胞中的游离体稳定性。
J Virol. 2012 Feb;86(3):1862-73. doi: 10.1128/JVI.06295-11. Epub 2011 Nov 30.
6
Production of recombinant Herpesvirus saimiri-based vectors.基于重组赛米利疱疹病毒载体的生产。
Cold Spring Harb Protoc. 2011 Dec 1;2011(12):1515-9. doi: 10.1101/pdb.prot066944.
7
Kaposi's Sarcoma-Associated Herpesvirus LANA-Adjacent Regions with Distinct Functions in Episome Segregation or Maintenance.卡波西肉瘤相关疱疹病毒 LANA 邻近区域在游离体分离或维持中具有不同的功能。
J Virol. 2019 Mar 5;93(6). doi: 10.1128/JVI.02158-18. Print 2019 Mar 15.
8
Assessment of infectivity using a Herpesvirus saimiri (HVS) recombinant that expresses HVS-GFP.使用表达猴疱疹病毒绿色荧光蛋白（HVS-GFP）的重组猴疱疹病毒（HVS）评估感染性。
Cold Spring Harb Protoc. 2011 Dec 1;2011(12):1520-3. doi: 10.1101/pdb.prot066951.
9
Primary structure of the herpesvirus saimiri genome.猴疱疹病毒基因组的一级结构。
J Virol. 1992 Aug;66(8):5047-58. doi: 10.1128/JVI.66.8.5047-5058.1992.
10
A role for herpesvirus saimiri orf14 in transformation and persistent infection.猴疱疹病毒14型开放阅读框14在转化和持续感染中的作用。
J Virol. 1998 Aug;72(8):6770-6. doi: 10.1128/JVI.72.8.6770-6776.1998.
引用本文的文献
1
G-Quadruplexes: More Than Just a Kink in Microbial Genomes.G-四链体：微生物基因组中的不只是一个扭结。
Trends Microbiol. 2019 Feb;27(2):148-163. doi: 10.1016/j.tim.2018.08.011. Epub 2018 Sep 14.
2
Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome.爱泼斯坦-巴尔病毒核抗原 EBNA-LP 对于转化初始 B 细胞是必不可少的，并有助于转录因子募集到病毒基因组。
PLoS Pathog. 2018 Feb 20;14(2):e1006890. doi: 10.1371/journal.ppat.1006890. eCollection 2018 Feb.
3
Genome-wide analysis of G-quadruplexes in herpesvirus genomes.疱疹病毒基因组中G-四链体的全基因组分析。
BMC Genomics. 2016 Nov 21;17(1):949. doi: 10.1186/s12864-016-3282-1.
4
Herpesvirus BACs: past, present, and future.疱疹病毒细菌人工染色体：过去、现在与未来
J Biomed Biotechnol. 2011;2011:124595. doi: 10.1155/2011/124595. Epub 2010 Oct 27.
本文引用的文献
1
Open reading frame 73 is required for herpesvirus saimiri A11-S4 episomal persistence.猴疱疹病毒A11-S4附加型持续存在需要开放阅读框73。
J Gen Virol. 2005 Oct;86(Pt 10):2703-2708. doi: 10.1099/vir.0.81230-0.
2
Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68.鉴定小鼠γ疱疹病毒68裂解性DNA复制和包装所需的顺式序列。
J Virol. 2004 Sep;78(17):9123-31. doi: 10.1128/JVI.78.17.9123-9131.2004.
3
ORF73 of murine herpesvirus-68 is critical for the establishment and maintenance of latency.鼠疱疹病毒68的开放阅读框73对于潜伏期的建立和维持至关重要。
J Gen Virol. 2003 Dec;84(Pt 12):3405-3416. doi: 10.1099/vir.0.19594-0.
4
Generation and precise modification of a herpesvirus saimiri bacterial artificial chromosome demonstrates that the terminal repeats are required for both virus production and episomal persistence.猿猴疱疹病毒细菌人工染色体的构建及精确修饰表明，末端重复序列对于病毒产生和游离型持续存在均是必需的。
J Gen Virol. 2003 Dec;84(Pt 12):3393-3403. doi: 10.1099/vir.0.19387-0.
5
ORF73 of herpesvirus Saimiri strain C488 tethers the viral genome to metaphase chromosomes and binds to cis-acting DNA sequences in the terminal repeats.松鼠猴疱疹病毒C488株的开放阅读框73将病毒基因组与中期染色体相连，并与末端重复序列中的顺式作用DNA序列结合。
J Virol. 2003 Dec;77(23):12494-506. doi: 10.1128/jvi.77.23.12494-12506.2003.
6
Functional delivery of large genomic DNA to human cells with a peptide-lipid vector.利用肽-脂质载体将大片段基因组DNA功能性递送至人细胞
J Gene Med. 2003 Oct;5(10):883-892. doi: 10.1002/jgm.420.
7
The terminal repeats and latency-associated nuclear antigen of herpesvirus saimiri are essential for episomal persistence of the viral genome.猴疱疹病毒的末端重复序列和潜伏相关核抗原对于病毒基因组的附加体持久性至关重要。
J Gen Virol. 2002 Sep;83(Pt 9):2269-2278. doi: 10.1099/0022-1317-83-9-2269.
8
Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mediates episome persistence through cis-acting terminal repeat (TR) sequence and specifically binds TR DNA.卡波西肉瘤相关疱疹病毒潜伏相关核抗原1通过顺式作用末端重复序列介导附加体持久性，并特异性结合末端重复序列DNA。
J Virol. 2001 Apr;75(7):3250-8. doi: 10.1128/JVI.75.7.3250-3258.2001.
9
A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coli.一种通过大肠杆菌中的同源重组在细菌人工染色体中产生点突变的新方法。
Nucleic Acids Res. 2001 Feb 1;29(3):E14. doi: 10.1093/nar/29.3.e14.
10
Annexation of the interchromosomal space during viral infection.病毒感染期间染色体间空间的吞并。
Nat Cell Biol. 2000 Sep;2(9):661-5. doi: 10.1038/35023615.
Zotero 插件