Uzunova P, Sokolova T Z, Galabova T, Peneva V, Taskov M, Ribarov S
Department of Physics and Biophysics, Medico-Biological Institute, Sofia, Bulgaria.
Cor Vasa. 1991;33(4):333-42.
The dynamics of lipid peroxidation in isoprenaline-induced myocardiopathy was studied in rats. Spontaneous and Fe(II)- catalyzed generation of malondialdehyde (MDA)-like products and chemiluminescence in the heart, liver and brain homogenates were measured. The increase in the interval between treatment of rats with isoprenaline and their killing up to 48 hours led to an increase in MDA content in the heart. Both spontaneous and Fe(II)-induced chemiluminescence also reached their maxima after 48 hours. These data show that well expressed lipid peroxidation in the rat heart occurs approximately 48 hours after isoprenaline application. Isoprenaline metabolization in the liver leads to generation of activated oxygen species which are able to induce lipid peroxidation in the presence of Fe(II). The treatment of rats with isoprenaline caused well-expressed lipid peroxidation in the brain. The maximum of this process occurred approximately 36 hours after isoprenaline application. The results show that both spontaneous and Fe(II)-induced chemiluminescence might be used for the estimation of lipid peroxidation in rat heart homogenates.
研究了异丙肾上腺素诱导的大鼠心肌病中脂质过氧化的动态变化。测定了心脏、肝脏和脑组织匀浆中丙二醛（MDA）样产物的自发产生和铁（II）催化产生以及化学发光。从用异丙肾上腺素处理大鼠到处死大鼠的间隔时间延长至48小时，导致心脏中MDA含量增加。自发的和铁（II）诱导的化学发光在48小时后也达到最大值。这些数据表明，在应用异丙肾上腺素约48小时后，大鼠心脏中发生了明显的脂质过氧化。肝脏中异丙肾上腺素的代谢导致活性氧的产生，在铁（II）存在的情况下，活性氧能够诱导脂质过氧化。用异丙肾上腺素处理大鼠会导致大脑中明显的脂质过氧化。这个过程的最大值出现在应用异丙肾上腺素约36小时后。结果表明，自发的和铁（II）诱导的化学发光都可用于评估大鼠心脏匀浆中的脂质过氧化。
