Antonenkov V D
Biokhimiia. 1991 Sep;56(9):1591-8.
The effect of normal rat liver cytosol on the level of Fe/ADP-ascorbate-induced lipid peroxidation in the total particulate fraction (mitochondria plus microsomes) has been studied. The intensity of lipid peroxidation was measured using the chemiluminescence technique and by malonic dialdehyde (MDA) production. Dialyzed cytosol significantly decreased the level of chemiluminescence and, to a much lesser extent, the rate of MDA production. Gel filtration on a Sephadex G-200 column led to the appearance of at least three cytosolic fractions which suppressed the low-level chemiluminescence. These fractions differed from one another by their molecular masses, kinetics of chemiluminescence inhibition and effects on the intensity of MDA production. The putative functional role of antioxidative defence factors from rat liver cytosol is discussed.
研究了正常大鼠肝细胞溶质对总颗粒组分（线粒体加微粒体）中Fe/抗坏血酸-ADP诱导的脂质过氧化水平的影响。使用化学发光技术并通过丙二醛（MDA）生成来测量脂质过氧化强度。透析后的细胞溶质显著降低了化学发光水平，且在小得多的程度上降低了MDA生成速率。在葡聚糖G-200柱上进行凝胶过滤，得到至少三个抑制低水平化学发光的细胞溶质组分。这些组分在分子量、化学发光抑制动力学以及对MDA生成强度的影响方面彼此不同。讨论了大鼠肝细胞溶质中抗氧化防御因子的假定功能作用。
