Yurugi K, Kimura S, Ashihara E, Tsuji H, Kawata A, Kamitsuji Y, Hishida R, Takegawa M, Egawa H, Maekawa T
Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto, Japan.
Transfus Med. 2007 Apr;17(2):97-106. doi: 10.1111/j.1365-3148.2007.00737.x.
High anti-blood group A or B (anti-A/B) immunoglobulin G (IgG) haemagglutination titres are associated with poor graft survival in ABO-unmatched liver transplantation. We have previously reported that the surface plasmon resonance (SPR) method can be used to measure anti-A/B IgG levels in the plasma very quickly and quantitatively. The aim of this study was to brush up this SPR method. The anti-A/B IgG antibodies (Abs) were purified from the plasma of healthy volunteers by affinity chromatography and used to establish standard curves for the SPR and flow cytometry (FCM) methods. The haemagglutination test tube (TT), FCM and SPR methods were then used to measure the changes over time in the anti-A/B IgG titres of 25 ABO-unmatched living donor liver transplantation (LDLT) recipients. The standard curve permitted the SPR values for the anti-A/B IgG titres to be expressed in microg mL(-1) units. The SPR measurements of the anti-A/B IgG levels in the LDLT recipients correlated very well with the FCM values, whereas the TT values correlated poorly with either method. Furthermore, the SPR method accurately detected the effects of plasma exchange. In conclusion, the SPR method is an accurate, time- and labour-saving method for measuring anti-A/B IgG titres that can be easily standardized.
高抗A或抗B血型（抗A/B）免疫球蛋白G（IgG）血凝滴度与ABO血型不匹配的肝移植中移植物存活率低相关。我们之前报道过表面等离子体共振（SPR）方法可用于非常快速且定量地测量血浆中的抗A/B IgG水平。本研究的目的是完善这种SPR方法。通过亲和层析从健康志愿者血浆中纯化抗A/B IgG抗体，并用于建立SPR和流式细胞术（FCM）方法的标准曲线。然后使用血凝试管（TT）、FCM和SPR方法来测量25例ABO血型不匹配的活体供肝肝移植（LDLT）受者抗A/B IgG滴度随时间的变化。该标准曲线使抗A/B IgG滴度的SPR值能够以微克每毫升（μg mL⁻¹）为单位表示。LDLT受者中抗A/B IgG水平的SPR测量值与FCM值相关性非常好，而TT值与这两种方法的相关性都很差。此外，SPR方法准确地检测到了血浆置换的效果。总之，SPR方法是一种准确、省时省力且易于标准化的测量抗A/B IgG滴度的方法。
