Krishnan Nithya S, Fleetwood Paul, Higgins Robert M, Hathaway Mark, Zehnder Daniel, Mitchell Daniel, Hamer Rizwan, Fletcher Simon, Lam For Tai, Kashi Habib, Tan Lam Chin, Imray Christopher, Briggs David
Renal Department, University Hospitals Coventry and Warwickshire NHS Trust, United Kingdom.
Transplantation. 2008 Aug 15;86(3):474-7. doi: 10.1097/TP.0b013e31817c4c4c.
Current methods of measuring ABO antibody levels based on the hemagglutination (HA) titers have the disadvantages of relatively poor reproducibility and do not offer fine discrimination of antibody concentration. We therefore developed a simple and rapid method of measuring ABO antibody levels using flow cytometry (FC). For validation, we analyzed plasma samples from 79 blood donors. Both IgM and IgG were detected and measured with IgG essentially restricted blood group O donors. Forty-two successive samples were collected from a patient with blood group O undergoing antibody removal and subsequent transplantation from a group A2 donor and tested by both HA and FC. Changes in IgG measured by FC (relative median fluorescence) correlated well with HA titers and importantly rejection episodes were preempted by a rising relative median fluorescence. The method allowed quantitative discrimination in the range of antibody levels relevant to ABO incompatible transplantation and has the advantages over HA of objective measurement and reproducibility.
当前基于血凝（HA）滴度测量ABO抗体水平的方法存在重复性相对较差的缺点，并且无法对抗体浓度进行精细区分。因此，我们开发了一种使用流式细胞术（FC）测量ABO抗体水平的简单快速方法。为了进行验证，我们分析了79名献血者的血浆样本。同时检测到了IgM和IgG，并且IgG主要在O型血供者中检测到。从一名O型血患者身上连续采集了42份样本，该患者正在接受抗体清除治疗，随后接受了来自A2型供者的移植，并通过HA和FC进行检测。通过FC测量的IgG变化（相对中位荧光）与HA滴度密切相关，重要的是，相对中位荧光的升高预示着排斥反应的发生。该方法能够在与ABO血型不相容移植相关的抗体水平范围内进行定量区分，并且与HA相比，具有客观测量和可重复性的优点。
