一个位于14个不同人类染色体末端亚端粒区域的低拷贝重复序列。

A low-copy repeat located in subtelomeric regions of 14 different human chromosomal termini.

作者信息

Weber B, Allen L, Magenis R E, Hayden M R

机构信息

Department of Medical Genetics, University of British Columbia, Vancouver, Canada.

出版信息

Cytogenet Cell Genet. 1991;57(4):179-83. doi: 10.1159/000133141.

DOI:10.1159/000133141

PMID:1743071

Abstract

Two cosmid clones, cG1A3 and cG1A6, containing large blocks of a telomeric repeat sequence, (TTAGGG)n, were isolated independently. Restriction mapping and fingerprinting studies suggested that cosmid cG1A6 is entirely contained within cG1A3. Competitive in situ hybridization localized cG1A3 to 14 different human telomeres and one internal site at 2q14----q21, indicating the presence of a subtelomeric low-copy repeat within this cosmid.

摘要

两个含有大片端粒重复序列（TTAGGG）n的黏粒克隆cG1A3和cG1A6被独立分离出来。限制性酶切图谱和指纹图谱研究表明黏粒cG1A6完全包含在cG1A3内。竞争性原位杂交将cG1A3定位到14个不同的人类端粒以及2q14----q21处的一个内部位点，表明该黏粒中存在亚端粒低拷贝重复序列。