Yan Chun-yan, Zhang Zhang, Yu Rong-min, Kong Ling-yi
College of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China.
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):192-5.
To study the biotransformation of arbutin by 4-hydroxy phenol in hairy root of Polygonum multiflorum.
4-hydroxy phenol was used as substrate, the standard curve was made by HPLC, and the influences of the co-culture time, the concentration of substrate added and the volume of culture flasks on biotransformation of arbutin were measured by the index of the production yield and transform rate of arbutin.
Arbutin could be detected from both of the cultures and medium. The correlation curve of arbutin: Y = 440740X - 1.473 (r = 0.9997). The production yield (2.22 g x L(-1)) and conversion ratio (81.45%) of arbutin reached the maximum amount as co-culture time at 72 h, substrate added in medium for 1100 mg x L(-1). Furthermore a large-scale culture of 3 L was also successful in our experiment.
It was firstly to biosynthesis arbutin in hairy root of P. multiflorum. The production yield and trasfer rate of arbutin were increased largely. And large-scale production (3 L culture flask) of arbutin was achieved in the experiment and it would be valuable for the industrial production of arbutin by biotechnological method in the future.
研究何首乌毛状根中4-羟基苯酚对熊果苷的生物转化作用。
以4-羟基苯酚为底物，采用高效液相色谱法绘制标准曲线，以熊果苷的产率和转化率为指标，考察共培养时间、底物添加浓度和培养瓶体积对熊果苷生物转化的影响。
在培养物和培养基中均能检测到熊果苷。熊果苷的相关曲线为：Y = 440740X - 1.473（r = 0.9997）。当共培养时间为72 h、培养基中添加底物1100 mg·L-1时，熊果苷的产率（2.22 g·L-1）和转化率（81.45%）达到最大值。此外，我们的实验中3 L的大规模培养也取得了成功。
首次在何首乌毛状根中生物合成熊果苷，熊果苷的产率和转化率大幅提高。实验实现了熊果苷的大规模生产（3 L培养瓶），这对未来采用生物技术方法工业化生产熊果苷具有重要价值。
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