Dong Quan-Feng, Jia Jun-Zhang, Zhu Jian-Hua, Yu Rong-Min
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2009 Oct;32(10):1495-9.
The exogenous substrate, thymol, was firstly biotransformed by using suspension hairy roots of transgenic Polygonum multflorum, and its biotransformed situation was also investigated.
After five days co-cultivated period, the transformed product was isolated by Thin Layer Chromatograph and Column Chromatograph, with the structure elucidated by physic-chemical methods and spectra data. Meanwhile, the time course of biotransformation (T-C) for thymol was also measured by HPLC to illuminate its bio-transformed situation.
The glycosylated product, namely DMP, was isolated and purified, which structure was determined as 5-methyl-2-(1-methylethyl) phenyl- beta-D-glucopyranoside. And the distribution of DMP in the medium or culture was varied in different co-cultivated periods, and for five days co-cultivated period, it mainly existed in the medium.
The hairy roots of Polygonum multiflorum were able to convert the aromatic exogenous substrate, thymol, into its glycoside. Furthermore, the time course indicated the relationship between DMP and co-cultivated period.
首次利用转基因何首乌悬浮毛状根对外源底物百里香酚进行生物转化，并研究其生物转化情况。
经过5天的共培养期后，通过薄层色谱和柱色谱分离转化产物，采用理化方法和光谱数据对其结构进行解析。同时，利用高效液相色谱法测定百里香酚的生物转化时间进程（T-C），以阐明其生物转化情况。
分离并纯化得到糖基化产物，即DMP，其结构确定为5-甲基-2-（1-甲基乙基）苯基-β-D-吡喃葡萄糖苷。在不同共培养时期，DMP在培养基或培养物中的分布有所不同，在5天共培养期内，它主要存在于培养基中。
何首乌毛状根能够将芳香族外源底物百里香酚转化为其糖苷。此外，时间进程表明了DMP与共培养期之间的关系。
Zotero 插件