高效液相色谱法测定复方苦参注射液中苦参碱、槐定碱和氧化苦参碱的含量
[Determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection by HPLC].
作者信息
Tian Juan, Wang Wei-hao, Gao Hui-min, Wang Zhi-min
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):222-4.
OBJECTIVE
To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection.
METHOD
Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm.
RESULT
The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively.
CONCLUSION
The method was simple, rapid, accurate and specific and suitable for quality control of the product.
目的
建立高效液相色谱法测定复方苦参注射液中苦参碱、槐定碱和氧化苦参碱的含量。
方法
采用Alltima氨基柱，以乙腈-3%磷酸-乙醇（80:10:10）为流动相，检测波长为220nm。
结果
复方苦参注射液中苦参碱、槐定碱和氧化苦参碱的平均回收率分别为99.51%（RSD 1.58%）、99.24%（RSD 1.44%）和100.22%（RSD 1.85%）。
结论
该方法简便、快速、准确、专属性强，适用于该产品的质量控制。
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