Sabir Gulnar, Aisa Haji Akber, Shi Ming-Hui, Jia Xiao-Guang
Xingjiang Institute of Chinese Material Medica and Ethica, Urumch 830002, China.
Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(24):2619-22.
To develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides.
Chromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.
All calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively.
This method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed.
建立高效液相色谱法同时测定苦豆子种子中槐定碱、氧化苦参碱和氧化槐果碱的含量。
色谱条件为使用菲罗门Gemini C18柱（4.6 mm×250 mm，5μm），流动相为甲醇-0.2%磷酸（7:93）混合溶液。检测波长为205 nm。流速为1.0 mL·min⁻¹，柱温设定为30℃。
各校准曲线在测试范围内均显示出良好的线性关系（r≥0.9997）。平均回收率分别为100.0%、97.0%和97.0%。
该方法简便、快速、准确，适用于苦豆子种子的质量控制。
