一株来自中国的猪捷申病毒1型分离株的分离与分子特征分析
Isolation and molecular characterization of a porcine teschovirus 1 isolate from China.
作者信息
Feng L, Shi H Y, Liu S W, Wu B P, Chen J F, Sun D B, Tong Y E, Fu M S, Wang Y F, Tong G Z
机构信息
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
出版信息
Acta Virol. 2007;51(1):7-11.
Porcine teschovirus 1 (PTV-1) (Swine/CH/IMH/03) was isolated from piglets in a farm in Inner Mongolia Province, P.R. China. It was confirmed by electron microscopy, RT-PCR, and sequencing. Comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein showed that PTV Swine/CH/IMH/03 strain is PTV-1. The isolated virus has closest relationship with Talfan strain, they shared 98.9% and 99.5% homology of amino acids and nucleotides, respectively, in the ORF of polyprotein. To our knowledge, this is the first report about isolation and identification of a PTV in China.
猪捷申病毒1型（PTV-1）（猪/中国/内蒙古/IMH/03株）从中国内蒙古自治区一个猪场的仔猪中分离得到。通过电子显微镜、逆转录聚合酶链反应（RT-PCR）和测序进行了确认。对氨基酸和核苷酸序列的比较以及多聚蛋白的系统发育分析表明，猪/中国/内蒙古/IMH/03株PTV为PTV-1。分离出的病毒与塔尔凡毒株关系最为密切，在多聚蛋白的开放阅读框中，它们的氨基酸和核苷酸同源性分别为98.9%和99.5%。据我们所知，这是中国关于PTV分离鉴定的首次报道。
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