Rogers Ian, Yamanaka Nobuko, Bielecki Ryszard, Wong Christine J, Chua Shawn, Yuen Shelia, Casper Robert F
Department of Obstetrics and Gynaecology, Rm. 876 Samuel Lunenfeld Research Institute, Mount Sinai Hospital and the University of Toronto, 600 University Ave, Toronto, Ontario, Canada M5G 1X5.
Exp Cell Res. 2007 May 15;313(9):1839-52. doi: 10.1016/j.yexcr.2007.02.029. Epub 2007 Mar 12.
We report on a subset of cells that co-purify with CD45-positive/Lineage minus (CD45(pos)/Lin(minus)) hematopoietic cells that are capable of in vitro differentiation into multi-potential cells including cells with neuroectoderm properties. Although these cells are CD45 positive and have properties similar to CD45-negative mesenchymal progenitor cells (MPC) derived from bone marrow (BM), they are neither hematopoietic cells nor mesenchymal cells. These CD45(pos)/Lin(minus) cells can be expanded in vitro, express the stem cell genes Oct-4 and Nanog and can be induced to differentiate into endothelial cells, osteoblasts, muscle cells and neural cells at frequencies similar to those reported for bone marrow mesenchymal cells. Long-term culture of these cells followed by transplantation into NOD/SCID mice resulted in positive bone marrow stromal cell engraftment but not hematopoietic engraftment, suggesting that despite their CD45-positive status these cells do not have the same properties as hematopoietic stem cells. Clonal cell analysis determined that the culture period caused a broadening in the differentiation potential of the starting population.
我们报告了一类与CD45阳性/谱系阴性（CD45(pos)/Lin(minus)）造血细胞共纯化的细胞亚群，这些造血细胞能够在体外分化为多能细胞，包括具有神经外胚层特性的细胞。尽管这些细胞CD45呈阳性，且具有与源自骨髓（BM）的CD45阴性间充质祖细胞（MPC）相似的特性，但它们既不是造血细胞也不是间充质细胞。这些CD45(pos)/Lin(minus)细胞可在体外扩增，表达干细胞基因Oct-4和Nanog，并能以与报道的骨髓间充质细胞相似的频率被诱导分化为内皮细胞、成骨细胞、肌肉细胞和神经细胞。对这些细胞进行长期培养后移植到NOD/SCID小鼠体内，结果显示骨髓基质细胞呈阳性植入，但造血细胞未植入，这表明尽管这些细胞CD45呈阳性，但其特性与造血干细胞不同。克隆细胞分析确定，培养期导致起始群体的分化潜能变宽。
