Fuchs Beate, Schober Celestina, Richter Grit, Süss Rosmarie, Schiller Jürgen
University of Leipzig, Faculty of Medicine, Institute of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany.
J Biochem Biophys Methods. 2007 Jun 10;70(4):689-92. doi: 10.1016/j.jbbm.2007.03.001. Epub 2007 Mar 13.
Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly applied to lipids. However, positional acyl chain analysis of lipids by MALDI was so far scarcely described. In this paper, the fragmentation behavior of phosphatidylethanolamine (PE) is investigated by using post-source decay (PSD) MS. In dependence on the investigated adduct, significant differences could be obtained. It will be shown that in particular the negative ion spectra enable the determination of the individual acyl chains as well as their positions (sn-1 or sn-2). Therefore, MALDI-TOF PSD spectra are a real alternative to more sophisticated MS/MS methods.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）越来越多地应用于脂质分析。然而，迄今为止，关于通过MALDI对脂质进行位置酰基链分析的报道很少。在本文中，通过使用源后衰变（PSD）质谱研究了磷脂酰乙醇胺（PE）的碎裂行为。根据所研究的加合物，可以获得显著差异。结果表明，特别是负离子谱能够确定单个酰基链及其位置（sn-1或sn-2）。因此，MALDI-TOF PSD谱是更复杂的MS/MS方法的一种切实可行的替代方法。
