Tilley Leann, McFadden Geoff, Cowman Alan, Klonis Nectarios
Department of Biochemistry, La Trobe University, Melbourne, Victoria 3086, Australia.
Trends Parasitol. 2007 Jun;23(6):268-77. doi: 10.1016/j.pt.2007.04.001. Epub 2007 Apr 16.
The malaria parasite undergoes a remarkable series of morphological transformations, which underpin its life in both human and mosquito hosts. The advent of molecular transfection technology coupled with the ability to introduce fluorescent reporter proteins that faithfully track and expose the activities of parasite proteins has revolutionized our view of parasite cell biology. The greatest insights have been realized in the erythrocyte stages of Plasmodium falciparum. P. falciparum invades and remodels the human erythrocyte: it feeds on haemoglobin, grows and divides, and subverts the physiology of its hapless host. Fluorescent proteins have been employed to track and dissect each of these processes and have revealed details and exposed new paradigms.
疟原虫经历了一系列显著的形态转变，这些转变是其在人类和蚊子宿主中生存的基础。分子转染技术的出现，以及引入能忠实地追踪和揭示寄生虫蛋白质活性的荧光报告蛋白的能力，彻底改变了我们对寄生虫细胞生物学的看法。在恶性疟原虫的红细胞阶段取得了最重大的见解。恶性疟原虫侵入并重塑人类红细胞：它以血红蛋白为食，生长并分裂，还会破坏其不幸宿主的生理机能。荧光蛋白已被用于追踪和剖析这些过程中的每一个，并揭示了细节，还展现了新的模式。
