Jandrositz A, Turnowsky F, Högenauer G
Institut für Mikrobiologie, Karl-Franzens-Universität Graz, Austria.
Gene. 1991 Oct 30;107(1):155-60. doi: 10.1016/0378-1119(91)90310-8.
The gene (ERG1) encoding squalene epoxidase (ERG) from Saccharomyces cerevisiae was cloned. It was isolated from a gene library, prepared from an allylamine-resistant (AlR) S. cerevisiae mutant, by screening transformants in a sensitive strain for AlR colonies. The ERG tested in a cell-free extract from one of these transformants proved to be resistant to the Al derivative, terbinafine. From this result, we concluded that the recombinant plasmid in the transformant carried an allelic form of the ERG1 gene. The nucleotide sequence showed the presence of one open reading frame coding for a 55,190-Da peptide of 496 amino acids. Southern hybridization experiments allowed us to localize the ERG1 gene on yeast chromosome 15.
克隆了酿酒酵母中编码角鲨烯环氧化酶（ERG）的基因（ERG1）。该基因是从一个由抗烯丙胺（AlR）的酿酒酵母突变体制备的基因文库中分离得到的，方法是在一个敏感菌株中筛选转化子以获得AlR菌落。在其中一个转化子的无细胞提取物中测试的ERG被证明对Al衍生物特比萘芬具有抗性。根据这一结果，我们得出结论，该转化子中的重组质粒携带了ERG1基因的等位形式。核苷酸序列显示存在一个开放阅读框，编码一个由496个氨基酸组成的55,190道尔顿的肽。Southern杂交实验使我们能够将ERG1基因定位在酵母第15号染色体上。
