人睾丸中 LIM 结构域家族基因 PINCH2 的一种新型剪接变体的分子克隆与鉴定。
Molecular cloning and characterization of a novel splice variant of the LIM domain family gene, PINCH 2, in human testis.
机构信息
Institute of Stomatology, Nanjing Medical University, 140 Han Zhong Road, Nanjing 210029, People's Republic of China.
出版信息
Mol Biotechnol. 2007 Feb;35(2):109-18. doi: 10.1007/BF02686105.
By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, PINCH 2. PINCH 2 expression was 3.4-fold higher in adult than in fetal testis. The full length of its cDNA was 963 bp, with a 354-bp open reading frame (ORF), encoding a 117-amino acid protein. PINCH 2 was a splicing isoform of PINCH. It shared one exon, which encoded the LIM domain, with PINCH gene in human genome. Multitissue reverse transcriptase-polymerase chain reaction (RTPCR) analysis revealed that this gene was expressed variably in a wide range of tissues, with high expression levels in human adult testis. These results suggest that PINCH 2, a novel LIM domain-containing gene, may play an important role in testicular development/spermatogenesis.
通过将人类成体睾丸 cDNA 微阵列与人类成体和胚胎睾丸 cDNA 探针杂交，我们鉴定了一个新的人类睾丸基因 PINCH2。PINCH2 在成体睾丸中的表达水平比在胎儿睾丸中高 3.4 倍。其 cDNA 的全长为 963bp，具有 354bp 的开放阅读框（ORF），编码一个 117 个氨基酸的蛋白质。PINCH2 是 PINCH 的剪接异构体。它与人类基因组中的 PINCH 基因共享一个外显子，该外显子编码 LIM 结构域。多组织逆转录-聚合酶链反应（RT-PCR）分析表明，该基因在广泛的组织中表达可变，在人成体睾丸中表达水平较高。这些结果表明，PINCH2 是一个新的 LIM 结构域基因，可能在睾丸发育/精子发生中发挥重要作用。
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