Mott Melissa L, Berger James M
Department of Molecular and Cell Biology, Quantitative Biology Institute, University of California, Berkeley, 237 Hildebrand Hall #3220, California 94720-3220, USA.
Nat Rev Microbiol. 2007 May;5(5):343-54. doi: 10.1038/nrmicro1640.
In all organisms, multi-subunit replicases are responsible for the accurate duplication of genetic material during cellular division. Initiator proteins control the onset of DNA replication and direct the assembly of replisomal components through a series of precisely timed protein-DNA and protein-protein interactions. Recent structural studies of the bacterial protein DnaA have helped to clarify the molecular mechanisms underlying initiator function, and suggest that key structural features of cellular initiators are universally conserved. Moreover, it appears that bacteria use a diverse range of regulatory strategies dedicated to tightly controlling replication initiation; in many cases, these mechanisms are intricately connected to the activities of DnaA at the origin of replication. This Review presents an overview of both the mechanism and regulation of bacterial DNA replication initiation, with emphasis on the features that are similar in eukaryotic and archaeal systems.
在所有生物体中，多亚基复制酶负责在细胞分裂期间精确复制遗传物质。起始蛋白控制DNA复制的起始，并通过一系列精确计时的蛋白质-DNA和蛋白质-蛋白质相互作用指导复制体组件的组装。最近对细菌蛋白DnaA的结构研究有助于阐明起始功能背后的分子机制，并表明细胞起始蛋白的关键结构特征是普遍保守的。此外，细菌似乎使用多种调控策略来严格控制复制起始；在许多情况下，这些机制与DnaA在复制起点的活性错综复杂地联系在一起。本综述概述了细菌DNA复制起始的机制和调控，重点介绍了真核生物和古细菌系统中相似的特征。
