Feng Zao-Ming, Fang Dian-Chun, Chen Wen-Sheng, Wang Rong-Quan
Department of Gastroenterology, Southwestern Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China.
Dig Dis Sci. 2007 Sep;52(9):2104-12. doi: 10.1007/s10620-006-9711-2. Epub 2007 Apr 12.
IgGFcgammaBP and TFF3 are related with adaptation during injury, mucosal defense, and epithelial healing. In this work, we produced the polyclonal antibodies for rat IgGFcgammaBP or TFF3 and assessed their tissue distributions in adult and prenatal rats, rTFF3 molecular patterns under reduced and nonreduced condition, involvement of IgGFcgammaBP, and TFF3 in dextran sulfate sodium (DSS)-induced colitis. Polyclonal antibodies of rat IgGFcgammaBP or TFF3 were produced with their synthetic polypeptide. Rat TFF3 was detected in the scraped intestinal mucosa by SDS/PAGE and Western blotting. Immunohistochemical stainings of rat IgGFcgammaBP or TFF3 were performed in different tissues, mainly in mucin-producing tissues, of adult rat and prenatal rat intestine. Rat IgGFcgammaBP and TFF3 were immunohistochemically detected in the distal colon of rat colitis model induced with 7% DSS. IgGFcgammaBP and TFF3 were mainly expressed in the intestinal mucosa with different distribution patterns. The scattered staining was also found in the epithelium of bile duct. There was strong expression of IgGFcgammaBP and TFF3 in rat embryonic intestine. There were two kinds of rTFF3 complexes existed with different molecular weights, 250 and 55 kDa, under nonreduced conditions, but shifted to 6 kDa under reduced conditions. In the DSS-induced colitis model, IgGFcgammaBP and TFF3 were significantly decreased in the distal colon mucosa at the onset and active phases comparing with the normal control, partially recovered at the regenerative phase. Based on these findings,IgGFcgammaBP and TFF3 were widely expressed in the intestinal mucosa, depleted during DSS-induced colitis. Rat TFF3 existed mainly in two complexes with 250 and 55 kDa molecular weights. The present findings indicate they are two important goblet cell-derived components possibly related to the pathogenesis of DSS-induced colitis, a rat model of ulcerative colitis.
免疫球蛋白G Fcγ结合蛋白（IgGFcgammaBP）和三叶因子3（TFF3）与损伤适应、黏膜防御及上皮愈合相关。在本研究中，我们制备了大鼠IgGFcgammaBP或TFF3的多克隆抗体，并评估了它们在成年和产前大鼠中的组织分布、还原和非还原条件下rTFF3的分子模式、IgGFcgammaBP和TFF3在葡聚糖硫酸钠（DSS）诱导的结肠炎中的作用。用合成多肽制备了大鼠IgGFcgammaBP或TFF3的多克隆抗体。通过SDS/PAGE和蛋白质印迹法在刮取的肠黏膜中检测到大鼠TFF3。在成年大鼠和产前大鼠肠道的不同组织（主要是产生黏蛋白的组织）中进行了大鼠IgGFcgammaBP或TFF3的免疫组织化学染色。在7% DSS诱导的大鼠结肠炎模型的远端结肠中通过免疫组织化学检测到大鼠IgGFcgammaBP和TFF3。IgGFcgammaBP和TFF3主要在肠黏膜中表达，分布模式不同。在胆管上皮中也发现了散在染色。大鼠胚胎肠道中有IgGFcgammaBP和TFF3的强表达。在非还原条件下存在两种不同分子量（250和55 kDa）的rTFF3复合物，但在还原条件下转变为6 kDa。在DSS诱导的结肠炎模型中，与正常对照相比，远端结肠黏膜在发病期和活动期IgGFcgammaBP和TFF3显著降低，在再生期部分恢复。基于这些发现，IgGFcgammaBP和TFF3在肠黏膜中广泛表达，在DSS诱导的结肠炎期间减少。大鼠TFF3主要以两种分子量为250和55 kDa的复合物形式存在。目前的研究结果表明它们是两个重要的杯状细胞衍生成分，可能与溃疡性结肠炎大鼠模型DSS诱导的结肠炎发病机制有关。
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