Department of Food and Nutrition, Sookmyung Women's University, Seoul, 140-742, Korea.
Dig Dis Sci. 2012 Feb;57(2):355-63. doi: 10.1007/s10620-011-1883-8. Epub 2011 Sep 8.
In ulcerative colitis (UC), reduction of inflammation may represent a key mechanism in UC therapy, and anti-inflammatory agents would be good candidates for preventing UC. Kaempferol, a natural flavonoid, is believed to have anti-inflammatory activities and has been shown to be potentially immune-modulatory.
The aim of this study was to determine whether kaempferol alleviates the inflammatory responses of dextran sulfate sodium (DSS)-induced colitis in mice.
Female C57BL/6J mice were divided into six groups: a negative control group, a DSS control group, and DSS + 0.1% or 0.3% kaempferol pre- or post-fed groups. At the end of the experimental period, clinical and biochemical markers were evaluated.
Plasma levels of NO and PGE(2) were significantly decreased in both the 0.3% kaempferol pre- and post-fed groups. The plasma LTB(4) level was profoundly decreased in all animals fed kaempferol. Colonic mucosa MPO activity was also suppressed in both the 0.3% kaempferol pre- or post-fed groups. TFF3 mRNA, a marker for goblet cell function, was up-regulated in kaempferol pre-fed animals.
These results indicate that kaempferol is an effective anti-inflammatory agent that protects colonic mucosa from DSS-induced UC. Dietary kaempferol fed prior to colitis induction was more effective to suppress some of the colitis-associated markers.
在溃疡性结肠炎（UC）中，炎症的减轻可能是 UC 治疗的关键机制，而抗炎药物将是预防 UC 的良好候选药物。山奈酚是一种天然类黄酮，被认为具有抗炎活性，并已显示出具有潜在的免疫调节作用。
本研究旨在确定山奈酚是否能减轻葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的炎症反应。
将雌性 C57BL/6J 小鼠分为六组：阴性对照组、DSS 对照组、DSS+0.1%或 0.3%山奈酚预饲或后饲组。在实验期末，评估临床和生化标志物。
0.3%山奈酚预饲和后饲组的血浆 NO 和 PGE（2）水平均显著降低。所有喂食山奈酚的动物的血浆 LTB（4）水平均明显降低。0.3%山奈酚预饲或后饲组的结肠黏膜 MPO 活性也受到抑制。TFF3mRNA，杯状细胞功能的标志物，在山奈酚预饲动物中上调。
这些结果表明，山奈酚是一种有效的抗炎剂，可保护结肠黏膜免受 DSS 诱导的 UC 损伤。在结肠炎诱导前给予饮食山奈酚更有效地抑制一些与结肠炎相关的标志物。
