一种用于检测血浆和尿液中3',5'-单磷酸腺苷的简单竞争性蛋白质结合测定法。
A simple competitive protein-binding assay for adenosine-3',5'-monophosphate in plasma and urine.
作者信息
Madsen S N, Badawi I, Skovsted L
出版信息
Acta Endocrinol (Copenh). 1976 Jan;81(1):208-14. doi: 10.1530/acta.0.0810208.
PMID:174364
Abstract
A modified competitive protein-binding assay for the measurement of adenosine-3',5'-monophosphate is described. The procedure allows measurement of adenosine-3',5'-monophosphate in unextracted plasma samples. The mean plasma values in 25 normal, fasting and ambulatory subjects were 22.7 +/- 4.7 pmol/ml (mean +/- SD) (range 13-31 pmol/ml. The mean urinary content was 3.2 +/- 1.0 mumol per g creatinine (mean +/- SD) (n=24).
摘要
本文描述了一种用于测量3',5'-环磷酸腺苷的改良竞争性蛋白结合测定法。该方法可对未提取的血浆样本中的3',5'-环磷酸腺苷进行测量。25名正常、空腹且可自由活动的受试者的血浆平均水平为22.7±4.7皮摩尔/毫升（平均值±标准差）（范围为13 - 31皮摩尔/毫升）。尿中平均含量为每克肌酐3.2±1.0微摩尔（平均值±标准差）（n = 24）。
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