Giulazian N M, Davtian T K, Pak S G
Klin Lab Diagn. 2007 Feb(2):20-3.
The differential sensitivity of peripheral blood granulocytes, monocytes, and lymphocytes to endotoxin-induced apoptosis was studied in convalescents with Salmonella infection. The number of early (Tunel+PI-) and late (Tunel+PI+) apoptotic cells and the rate of apoptosis (the relative content of DNA apoptotic fragments in the solitary cell) were determined on a FACSCalibur cytofluorometer, by applying a package of the CellQuest programs (Becton Dickinson). A higher threshold of sensitivity of granulocytes, monocytes, and lymphocytes to the apoptotic effect of low, suboptimal doses (100 ng), as well as suppression of early stages of apoptosis by the optimal doses (1000 ng) of S. enteritidis lypopolysaccharides (LPS) were observed in patients who had sustained salmonellosis at a stage of reconvalescence. The hierarchy of LPS-induced apoptosis (granulocyte--monocyte--lymphocyte), observed in healthy donors, was also retained in the group of patients receiving the suboptimal doses of LPS, which seems to be associated with the repeated action of toxin on sensitized cell populations.
在沙门氏菌感染恢复期患者中，研究了外周血粒细胞、单核细胞和淋巴细胞对内毒素诱导凋亡的差异敏感性。通过应用CellQuest程序包（BD公司），在FACSCalibur细胞荧光仪上测定早期（Tunel+PI-）和晚期（Tunel+PI+）凋亡细胞的数量以及凋亡率（单个细胞中DNA凋亡片段的相对含量）。在恢复期曾患沙门氏菌病的患者中，观察到粒细胞、单核细胞和淋巴细胞对低剂量、次优剂量（100 ng）凋亡作用的敏感性阈值较高，以及肠炎沙门氏菌脂多糖（LPS）的最佳剂量（1000 ng）对凋亡早期阶段的抑制作用。在健康供体中观察到的LPS诱导凋亡的等级顺序（粒细胞-单核细胞-淋巴细胞），在接受次优剂量LPS的患者组中也得以保留，这似乎与毒素对致敏细胞群体的反复作用有关。
