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[基于重组抗原EgF的酶免疫分析系统对绵羊幼虫棘球蚴病(包虫病)的诊断效能]

[Diagnostic efficiency of enzyme immunoassay system based on the recombinant antigen EgF in sheep larval hydatid disease (echinococcosis)].

作者信息

Odoevskaia I M

出版信息

Med Parazitol (Mosk). 2007 Jan-Mar(1):20-4.

PMID:17436725
Abstract

The paper presents the results of designing a technology for the manufacture of enzyme immunoassay kits to detect class G antibodies to the Echinococcus granulosus antigen EgF with a molecular weight of 16.7 kDa in sheep. According to the results of field trials on flocks of sheep from various age groups, the diagnostic efficiency of the test system was 78.1% sensitivity and 73.9% specificity. The proposed test system may be used during mass sheep screenings for the epizootic monitoring of the prevalence ofechinococcosis in the Russian Federation, for the serological diagnosis of this disease in the female livestock at the level of a flock.

摘要

本文介绍了一种用于制造酶免疫分析试剂盒的技术成果,该试剂盒用于检测绵羊体内针对细粒棘球绦虫抗原EgF(分子量为16.7 kDa)的G类抗体。根据对不同年龄组羊群的现场试验结果,该检测系统的诊断效率为灵敏度78.1%,特异性73.9%。所提出的检测系统可用于俄罗斯联邦绵羊群体大规模筛查,以进行棘球蚴病流行情况的动物疫情监测,以及在羊群层面上对雌性家畜进行该疾病的血清学诊断。

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Med Parazitol (Mosk). 2007 Jan-Mar(1):20-4.
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