Poh C L, Ocampo J C, Sng E H, Bygdeman S M
Department of Microbiology, Faculty of Medicine, National University of Singapore.
Genitourin Med. 1991 Oct;67(5):389-93. doi: 10.1136/sti.67.5.389.
To characterise Neisseria gonorrhoeae isolates from Singapore.
Characterisation of Neisseria gonorrhoeae isolates by auxotyping, serological analysis and plasmid profile analysis.
Sixty randomly collected isolates from 41 symptomatic, untreated males and 19 female prostitutes were studied.
Auxotyping of 25 PPNG and 35 non-PPNG strains showed that the Pro-auxotype was prevalent among both PPNG (56%) and non-PPNG (42.5%) strains. Prototrophic strains comprised 28% of PPNG and 32.5% of non-PPNG strains respectively. Serovar analysis showed that with the exception of seven serogroup WI strains, the majority belonged to serogroup WII/III. Serovar Aedih was predominant among both serogroup WI PPNG (80%) and non-PPNG (100%) strains. Serogroup WII/III PPNG strains were represented by nine serovars with the predominant serovars being Bacjk (28%) and Bcgjk (16%). Eleven serovars were identified in the WII/III non-PPNG strains and the major serovars were Bajk (20%), Bacjk (17%), Back (11.4%) and Beghjk (11.4%). Analysis of the 25 PPNG strains showed that 16 of them carried the 4.4 MDa (Asian type) resistance plasmid and nine strains harboured the 4.4 MDa plasmid in conjunction with the 24.5 MDa transfer plasmid. The cryptic plasmid of 2.6 MDa was present in 27 of the 35 non-PPNG strains. Five of the non-PPNG strains harbouring the cryptic plasmid also contained the 24.5 MDa transfer plasmid. The plasmid combination of 2.6 + 7.8 + 24.5 MDa was detected in three non-PPNG strains.
The combination of epidemiological methods used in this study indicated the heterogeneity of N gonorrhoeae strains in Singapore. A total of 16 different combinations of auxotype, plasmid profile and serovar were seen in the 25 PPNG strains compared with 24 such combinations in the 35 non-PPNG strains. Such sensitive differentiation would otherwise not be possible using either auxotype-serovar (A/S) or auxotype-plasmid analysis.
对来自新加坡的淋病奈瑟菌分离株进行特征分析。
通过辅助型别鉴定、血清学分析和质粒图谱分析对淋病奈瑟菌分离株进行特征分析。
对从41名有症状、未经治疗的男性和19名女性性工作者中随机收集的60株分离株进行研究。
对25株产青霉素酶淋病奈瑟菌（PPNG）和35株非PPNG菌株进行辅助型别鉴定显示，原养型在PPNG菌株（56%）和非PPNG菌株（42.5%）中均较为普遍。原养型菌株分别占PPNG菌株的28%和非PPNG菌株的32.5%。血清型分析表明，除7株血清群WI菌株外，大多数菌株属于血清群WII/III。血清型Aedih在血清群WI的PPNG菌株（80%）和非PPNG菌株（100%）中均占主导地位。血清群WII/III的PPNG菌株由9种血清型代表，其中主要血清型为Bacjk（28%）和Bcgjk（16%）。在血清群WII/III的非PPNG菌株中鉴定出11种血清型，主要血清型为Bajk（20%）、Bacjk（17%）、Back（11.4%）和Beghjk（11.4%）。对25株PPNG菌株的分析表明，其中16株携带4.4 MDa（亚洲型）耐药质粒，9株同时携带4.4 MDa质粒和24.5 MDa转移质粒。2.6 MDa的隐蔽质粒存在于35株非PPNG菌株中的27株。5株携带隐蔽质粒的非PPNG菌株还含有24.5 MDa转移质粒。在3株非PPNG菌株中检测到2.6 + 7.8 + 24.5 MDa的质粒组合。
本研究中使用的流行病学方法组合表明新加坡淋病奈瑟菌菌株具有异质性。在25株PPNG菌株中总共观察到16种不同的辅助型别、质粒图谱和血清型组合，而在35株非PPNG菌株中有24种此类组合。否则，使用辅助型别 - 血清型（A/S）或辅助型别 - 质粒分析不可能进行如此敏感的区分。
