Suganuma Nobuyasu, Segade Fernando, Matsuzu Kenichi, Bowden Donald W
Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
BJU Int. 2007 May;99(5):1143-9. doi: 10.1111/j.1464-410X.2007.06765.x.
To investigate the differences in the pattern of glucose transporter (GLUT) gene expression between normal and tumour tissues and among histological subtypes of renal cell carcinomas (RCCs), as malignant cells are characterized by increased glucose uptake and use.
Enhanced glucose uptake probably depends on the overexpression of GLUT, usually GLUT1 and/or GLUT3, but there are few comprehensive studies to evaluate the relative expression pattern and level of GLUT in normal and tumour kidney tissues, especially of the recently identified GLUT genes. In all, 71 kidney surgical samples were evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR) for GLUT1-14 in normal and tumour (clear cell, papillary and chromophobe RCC, and oncocytoma) tissues. The expression levels for GLUT1-5, 9, 10 and 12 were quantified by real-time quantitative PCR.
The RT-PCR results showed that normal kidney tissue expresses all the GLUT isoforms. In clear cell RCC GLUT1 expression increased (P<0.001) while GLUT4, 9 and 12 decreased (P<0.001). In papillary RCC there were no significant increases in GLUT expression, with only GLUT12 significantly expressed at lower levels (P<0.001). In chromophobe RCC the expression of GLUT4 increased (P<0.05), and GLUT2 and 5 decreased (P<0.01), whereas in oncocytoma tissue there were no significant changes in the expression of GLUT1 (P<0.01), 2, 5, 9 (P<0.001) and 10 (P<0.05).
These results suggest that high-affinity GLUTs might have a major role in enhanced glucose uptake in kidney tumours, and that histopathological types are characterized by specific patterns of GLUT expression.
鉴于恶性细胞具有葡萄糖摄取和利用增加的特征，研究正常组织与肿瘤组织之间以及肾细胞癌（RCC）组织学亚型之间葡萄糖转运蛋白（GLUT）基因表达模式的差异。
葡萄糖摄取增加可能依赖于GLUT的过表达，通常是GLUT1和/或GLUT3，但很少有综合研究评估正常和肿瘤肾脏组织中GLUT的相对表达模式和水平，尤其是最近发现的GLUT基因。总共71份肾脏手术样本通过逆转录聚合酶链反应（RT-PCR）评估正常和肿瘤（透明细胞、乳头状和嫌色性RCC以及嗜酸细胞瘤）组织中GLUT1-14的表达。通过实时定量PCR对GLUT1-5、9、10和12的表达水平进行定量。
RT-PCR结果显示正常肾脏组织表达所有GLUT异构体。在透明细胞RCC中，GLUT1表达增加（P<0.001），而GLUT4、9和12表达降低（P<0.001）。在乳头状RCC中，GLUT表达无显著增加，只有GLUT12表达显著降低（P<0.001）。在嫌色性RCC中，GLUT4表达增加（P<0.05），GLUT2和5表达降低（P<0.01），而在嗜酸细胞瘤组织中，GLUT1（P<0.01）、2、5、9（P<0.001）和10（P<0.05）的表达无显著变化。
这些结果表明，高亲和力GLUT可能在肾肿瘤葡萄糖摄取增加中起主要作用，并且组织病理学类型具有特定的GLUT表达模式。
