Hunger Robert E, Brönnimann Marcel, Kappeler Andreas, Mueller Christoph, Braathen Lasse R, Yawalkar Nikhil
Department of Dermatology, University of Bern, Inselspital, 3010 Bern, Switzerland.
Exp Dermatol. 2007 May;16(5):416-20. doi: 10.1111/j.1600-0625.2007.00543.x.
Granzyme B and perforin messenger RNA (mRNA) expression has been shown to be a specific in vivo activation marker for cytotoxic cells. The aim of this study was to assess the contribution of cell-mediated cytotoxicity in the pathogenesis of lichen sclerosus. In situ hybridization and immunohistochemistry were performed on serial tissue sections of lesional skin biopsies and normal skin as control. Immunohistochemical staining showed that the cellular infiltrate of diseased skin consisted predominantly of T cells (CD3+) and some B cells (CD20+). Among T cells CD4+ and CD8+ cells were found in about equal numbers. In normal skin samples perforin and granzyme B mRNA expressing cells were only rarely found. In contrast, in biopsies from diseased skin a high percentage of infiltrating cells expressed mRNA for perforin and granzyme B. The perforin and granzyme B expressing cells were found in the dermal infiltrate and intraepidermally in close proximity to keratinocytes suggesting in situ activation of these cells. These findings provide evidence that cell-mediated cytotoxicity plays a significant role in tissue destruction in lichen sclerosus.
颗粒酶B和穿孔素信使核糖核酸（mRNA）的表达已被证明是细胞毒性细胞的一种特异性体内激活标志物。本研究的目的是评估细胞介导的细胞毒性在硬化性苔藓发病机制中的作用。对病变皮肤活检组织的连续切片和作为对照的正常皮肤进行原位杂交和免疫组织化学检测。免疫组织化学染色显示，病变皮肤的细胞浸润主要由T细胞（CD3+）和一些B细胞（CD20+）组成。在T细胞中，CD4+和CD8+细胞的数量大致相等。在正常皮肤样本中，仅很少发现表达穿孔素和颗粒酶B mRNA的细胞。相比之下，在病变皮肤活检组织中，高比例的浸润细胞表达穿孔素和颗粒酶B的mRNA。表达穿孔素和颗粒酶B的细胞见于真皮浸润中以及表皮内紧邻角质形成细胞处，提示这些细胞的原位激活。这些发现提供了证据，表明细胞介导的细胞毒性在硬化性苔藓的组织破坏中起重要作用。
