• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.细菌人工染色体介导的免疫细胞靶向转基因技术——从细菌人工染色体到免疫学
Immunology. 2007 Jul;121(3):308-13. doi: 10.1111/j.1365-2567.2007.02605.x. Epub 2007 Apr 16.
2
Targeting iCre expression to murine progesterone receptor cell-lineages using bacterial artificial chromosome transgenesis.利用细菌人工染色体转基因技术将iCre表达靶向鼠孕激素受体细胞谱系。
Genesis. 2006 Dec;44(12):601-10. doi: 10.1002/dvg.20257.
3
Transgenic mice for conditional gene manipulation in astroglial cells.用于星形胶质细胞中条件性基因操作的转基因小鼠。
Glia. 2007 Nov 15;55(15):1565-76. doi: 10.1002/glia.20570.
4
Dkk3-Cre BAC transgenic mouse line: a tool for highly efficient gene deletion in retinal progenitor cells.Dkk3-Cre细菌人工染色体转基因小鼠品系：一种用于高效敲除视网膜祖细胞中基因的工具。
Genesis. 2007 Aug;45(8):502-7. doi: 10.1002/dvg.20318.
5
BAC to degeneration bacterial artificial chromosome (BAC)-mediated transgenesis for modeling basal ganglia neurodegenerative disorders.BAC 到退行性细菌人工染色体 (BAC)-介导的基因转染用于建模基底神经节神经退行性疾病。
Int Rev Neurobiol. 2009;89:37-56. doi: 10.1016/S0074-7742(09)89002-8.
6
Teaching molecular genetics: chapter 2-Transgenesis and gene targeting: mouse models to study gene function and expression.分子遗传学教学：第2章——转基因与基因靶向：用于研究基因功能和表达的小鼠模型
Pediatr Nephrol. 2006 Mar;21(3):318-23. doi: 10.1007/s00467-005-2110-7. Epub 2006 Jan 5.
7
Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome.基于同源重组对大肠杆菌进行修饰以及细菌人工染色体在转基因小鼠中的种系传递。
Nat Biotechnol. 1997 Sep;15(9):859-65. doi: 10.1038/nbt0997-859.
8
Bacterial artificial chromosome transgenesis for zebrafish.用于斑马鱼的细菌人工染色体转基因技术。
Methods Mol Biol. 2009;546:103-16. doi: 10.1007/978-1-60327-977-2_7.
9
Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection.通过胞质内精子注射生产携带大（>200 kb）外源DNA片段的近交系和杂交转基因小鼠。
Mol Reprod Dev. 2005 Nov;72(3):329-35. doi: 10.1002/mrd.20319.
10
Engineering the mouse genome to model human disease for drug discovery.通过改造小鼠基因组来模拟人类疾病以进行药物研发。
Methods Mol Biol. 2010;602:55-77. doi: 10.1007/978-1-60761-058-8_4.

引用本文的文献

1
Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones.脾脏中 T 细胞迁移的可视化显示了一个血管周途径网络，该网络指导 T 区进入。
Immunity. 2020 May 19;52(5):794-807.e7. doi: 10.1016/j.immuni.2020.03.010. Epub 2020 Apr 15.
2
Endogenous Expression of the Human CD83 Attenuates EAE Symptoms in Humanized Transgenic Mice and Increases the Activity of Regulatory T Cells.内源性表达人 CD83 可减轻人源化转基因小鼠的 EAE 症状并增加调节性 T 细胞的活性。
Front Immunol. 2019 Jun 25;10:1442. doi: 10.3389/fimmu.2019.01442. eCollection 2019.
3
Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.纤维母细胞网状细胞在脾脏白髓中的起源和分化轨迹。
Nat Commun. 2019 Apr 15;10(1):1739. doi: 10.1038/s41467-019-09728-3.
4
The Third Intron of the Interferon Regulatory Factor-8 Is an Initiator of Repressed Chromatin Restricting Its Expression in Non-Immune Cells.干扰素调节因子8的第三个内含子是抑制性染色质的启动子，限制其在非免疫细胞中的表达。
PLoS One. 2016 Jun 3;11(6):e0156812. doi: 10.1371/journal.pone.0156812. eCollection 2016.
5
RNA recognition by human TLR8 can lead to autoimmune inflammation.人 TLR8 对 RNA 的识别可导致自身免疫性炎症。
J Exp Med. 2013 Dec 16;210(13):2903-19. doi: 10.1084/jem.20131044. Epub 2013 Nov 25.
6
Application of a bacterial artificial chromosome modification system for a human artificial chromosome vector.细菌人工染色体修饰系统在人类人工染色体载体中的应用。
Yonago Acta Med. 2011 Mar;54(1):21-31. Epub 2011 Mar 1.
7
Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells.Foxp3+ 调节性 T 细胞中荧光素酶报告基因表达的变化。
PLoS One. 2012;7(8):e41971. doi: 10.1371/journal.pone.0041971. Epub 2012 Aug 6.
8
A novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo.一种新型细菌人工染色体转染的 podoplanin-cre 小鼠在体内靶向淋巴器官基质细胞。
Front Immunol. 2011 Oct 12;2:50. doi: 10.3389/fimmu.2011.00050. eCollection 2011.
9
In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice.在增强型 GFP-BAC 转基因小鼠中分析胃泌素基因调控的体内分析。
Am J Physiol Gastrointest Liver Physiol. 2011 Feb;300(2):G334-44. doi: 10.1152/ajpgi.00134.2010. Epub 2010 Nov 4.
10
Dynamic DNA methylation patterns across the mouse and human IL10 genes during CD4+ T cell activation; influence of IL-27.CD4+ T 细胞活化过程中鼠类和人类白细胞介素 10 基因的动态 DNA 甲基化模式；白细胞介素 27 的影响。
Mol Immunol. 2010 Nov-Dec;48(1-3):73-81. doi: 10.1016/j.molimm.2010.09.009. Epub 2010 Oct 16.

本文引用的文献

1
Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease.Foxp3+调节性T细胞的选择性耗竭会诱发类似头屑样的疾病。
J Exp Med. 2007 Jan 22;204(1):57-63. doi: 10.1084/jem.20061852. Epub 2007 Jan 2.
2
Disseminated and sustained HIV infection in CD34+ cord blood cell-transplanted Rag2-/-gamma c-/- mice.CD34+ 脐带血细胞移植的Rag2-/-γc-/-小鼠中HIV的播散性持续感染
Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15951-6. doi: 10.1073/pnas.0604493103. Epub 2006 Oct 12.
3
Epidermal langerhans cell-deficient mice develop enhanced contact hypersensitivity.表皮朗格汉斯细胞缺陷型小鼠发生增强的接触性超敏反应。
Immunity. 2005 Dec;23(6):611-20. doi: 10.1016/j.immuni.2005.10.008.
4
Measles virus replication in lymphatic cells and organs of CD150 (SLAM) transgenic mice.麻疹病毒在CD150（信号淋巴细胞激活分子）转基因小鼠的淋巴细胞和器官中的复制。
Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16415-20. doi: 10.1073/pnas.0505945102. Epub 2005 Oct 31.
5
The TCRbeta enhancer is dispensable for the expression of rearranged TCRbeta genes in thymic DN2/DN3 populations but not at later stages.TCRβ增强子对于胸腺中双阴性2/双阴性3（DN2/DN3）群体中重排的TCRβ基因的表达并非必需，但在后期阶段则不可或缺。
J Immunol. 2005 Sep 1;175(5):3067-74. doi: 10.4049/jimmunol.175.5.3067.
6
The 3' end of the heavy chain constant region locus enhances germline transcription and switch recombination of the four gamma genes.重链恒定区基因座的3'端增强了四个γ基因的种系转录和类别转换重组。
J Exp Med. 2005 May 2;201(9):1459-66. doi: 10.1084/jem.20041988. Epub 2005 Apr 25.
7
Natural regulatory T cells in infectious disease.传染病中的天然调节性T细胞。
Nat Immunol. 2005 Apr;6(4):353-60. doi: 10.1038/ni1181.
8
Germline transcription and switch recombination of a transgene containing the entire H chain constant region locus: effect of a mutation in a STAT6 binding site in the gamma 1 promoter.包含完整重链恒定区基因座的转基因的种系转录和类别转换重组：γ1启动子中STAT6结合位点突变的影响
J Immunol. 2004 Nov 1;173(9):5531-9. doi: 10.4049/jimmunol.173.9.5531.
9
The European dimension for the mouse genome mutagenesis program.小鼠基因组诱变计划的欧洲层面
Nat Genet. 2004 Sep;36(9):925-7. doi: 10.1038/ng0904-925.
10
The knockout mouse project.基因敲除小鼠项目。
Nat Genet. 2004 Sep;36(9):921-4. doi: 10.1038/ng0904-921.

细菌人工染色体介导的免疫细胞靶向转基因技术——从细菌人工染色体到免疫学

BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.

作者信息

Sparwasser Tim, Eberl Gérard

机构信息

Institut für Medizinische Mikrobiologie, Immunologie & Hygiene, Technische Universität München, Munich, Germany.

出版信息

Immunology. 2007 Jul;121(3):308-13. doi: 10.1111/j.1365-2567.2007.02605.x. Epub 2007 Apr 16.

DOI:10.1111/j.1365-2567.2007.02605.x
PMID:17437533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2265958/
Abstract

Thirty years after the first transgenic mouse was produced, a plethora of genetic tools has been developed to study immune cells in vivo. A powerful development is the bacterial artificial chromosome (BAC) transgenic approach, combining advantages of both conventional transgenic and knock-in gene-targeting strategies. In immunology the potential of BAC transgenic technology has yet to be fully harvested and, combined with a variety of elegant genetic tools, it will allow the analysis of complex immunological processes in vivo. In this short review, we discuss the applications of BACs in immunology, such as identification of regulatory regions, expression and cell-fate mapping, cell ablation, conditional mutations and the generation of humanized mice.

摘要

在第一只转基因小鼠诞生30年后，已经开发出大量遗传工具用于在体内研究免疫细胞。一项重大进展是细菌人工染色体（BAC）转基因方法，它结合了传统转基因和基因敲入靶向策略的优点。在免疫学领域，BAC转基因技术的潜力尚未得到充分挖掘，与各种精妙的遗传工具相结合，它将使体内复杂免疫过程的分析成为可能。在这篇简短的综述中，我们讨论了BAC在免疫学中的应用，如调控区域的鉴定、表达和细胞命运图谱分析、细胞消融、条件性突变以及人源化小鼠的产生。