细菌人工染色体介导的免疫细胞靶向转基因技术——从细菌人工染色体到免疫学
BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.
作者信息
Sparwasser Tim, Eberl Gérard
机构信息
Institut für Medizinische Mikrobiologie, Immunologie & Hygiene, Technische Universität München, Munich, Germany.
出版信息
Immunology. 2007 Jul;121(3):308-13. doi: 10.1111/j.1365-2567.2007.02605.x. Epub 2007 Apr 16.
Abstract
Thirty years after the first transgenic mouse was produced, a plethora of genetic tools has been developed to study immune cells in vivo. A powerful development is the bacterial artificial chromosome (BAC) transgenic approach, combining advantages of both conventional transgenic and knock-in gene-targeting strategies. In immunology the potential of BAC transgenic technology has yet to be fully harvested and, combined with a variety of elegant genetic tools, it will allow the analysis of complex immunological processes in vivo. In this short review, we discuss the applications of BACs in immunology, such as identification of regulatory regions, expression and cell-fate mapping, cell ablation, conditional mutations and the generation of humanized mice.
摘要
在第一只转基因小鼠诞生30年后，已经开发出大量遗传工具用于在体内研究免疫细胞。一项重大进展是细菌人工染色体（BAC）转基因方法，它结合了传统转基因和基因敲入靶向策略的优点。在免疫学领域，BAC转基因技术的潜力尚未得到充分挖掘，与各种精妙的遗传工具相结合，它将使体内复杂免疫过程的分析成为可能。在这篇简短的综述中，我们讨论了BAC在免疫学中的应用，如调控区域的鉴定、表达和细胞命运图谱分析、细胞消融、条件性突变以及人源化小鼠的产生。
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