Austin Christopher P, Battey James F, Bradley Allan, Bucan Maja, Capecchi Mario, Collins Francis S, Dove William F, Duyk Geoffrey, Dymecki Susan, Eppig Janan T, Grieder Franziska B, Heintz Nathaniel, Hicks Geoff, Insel Thomas R, Joyner Alexandra, Koller Beverly H, Lloyd K C Kent, Magnuson Terry, Moore Mark W, Nagy Andras, Pollock Jonathan D, Roses Allen D, Sands Arthur T, Seed Brian, Skarnes William C, Snoddy Jay, Soriano Philippe, Stewart David J, Stewart Francis, Stillman Bruce, Varmus Harold, Varticovski Lyuba, Verma Inder M, Vogt Thomas F, von Melchner Harald, Witkowski Jan, Woychik Richard P, Wurst Wolfgang, Yancopoulos George D, Young Stephen G, Zambrowicz Brian
National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA.
Nat Genet. 2004 Sep;36(9):921-4. doi: 10.1038/ng0904-921.
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.
小鼠基因敲除技术为在体内阐明基因功能提供了一种强大的手段，而公开可用的全基因组小鼠基因敲除文库将极大地推动生物医学发现。迄今为止，已发表的基因敲除仅涉及约10%的小鼠基因。此外，其中许多基因敲除的实用性有限，因为它们并非以标准化方式构建或进行表型分析，而且许多资源研究人员无法免费获取。现在是时候利用新技术和提高生产效率，开展一项高通量的国际合作，为所有小鼠基因生产基因敲除并进行表型分析，并将这些资源置于公共领域了。
