Lee Hyun Kyoung, Seo In Ae, Park Hye Kyung, Park Yoo Mi, Ahn Kyoung Jin, Yoo Young Hyun, Park Hwan Tae
Department of Physiology, Medical Science Research Institute, College of Medicine, Dong-A University, Busan, South Korea.
J Neurochem. 2007 Aug;102(3):686-98. doi: 10.1111/j.1471-4159.2007.04580.x. Epub 2007 Apr 16.
Schwann cells provide a favorable microenvironment for successful regeneration of the injured peripheral nerve. Even though the roles of extracellular matrix proteins in the Schwann cell physiology have long been studied, the precise function of nidogen, a ubiquitous component of the basal lamina, in Schwann cells is unknown. In this study, we show that the protein and mRNA messages for nidogens are up-regulated in the sciatic nerve after sciatic nerve transection. We demonstrate that recombinant nidogen-1 increased the process formation of Schwann cells cultured from adult rat sciatic nerves and that nidogen-1 prevented Schwann cells from serum-deprivation-induced death. In addition, nidogen-1 promoted spontaneous migration of Schwann cells in two-independent migration assays. The Schwann cell responses to the recombinant nidogen-1 were specific because the nidogen-binding ectodomain of tumor endothelial marker 7 inhibited the nidogen responses without affecting Schwann cell response to laminin. Finally, we found that beta1 subunit-containing integrins play a key role in the nidogen-induced process formation, survival, and migration of Schwann cells. Altogether, these results indicate that nidogen has a prosurvival and promigratory activity on Schwann cells in the peripheral nerve.
施万细胞为受损周围神经的成功再生提供了有利的微环境。尽管细胞外基质蛋白在施万细胞生理学中的作用早已得到研究，但基底膜中普遍存在的巢蛋白在施万细胞中的精确功能仍不清楚。在本研究中，我们发现坐骨神经横断后，坐骨神经中巢蛋白的蛋白质和mRNA表达上调。我们证明重组巢蛋白-1增加了成年大鼠坐骨神经培养的施万细胞的突起形成，并且巢蛋白-1可防止施万细胞因血清剥夺诱导的死亡。此外，在两项独立的迁移试验中，巢蛋白-1促进了施万细胞的自发迁移。施万细胞对重组巢蛋白-1的反应具有特异性，因为肿瘤内皮标志物7的巢蛋白结合胞外域抑制了巢蛋白反应，而不影响施万细胞对层粘连蛋白的反应。最后，我们发现含β1亚基的整合素在巢蛋白诱导的施万细胞突起形成、存活和迁移中起关键作用。总之，这些结果表明巢蛋白对周围神经中的施万细胞具有促存活和促迁移活性。
