Sayin T Cem, Serper Ahmet, Cehreli Zafer C, Kalayci Sukru
Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
J Endod. 2007 May;33(5):581-4. doi: 10.1016/j.joen.2006.12.010. Epub 2007 Mar 12.
The aim of this study was to determine the extent of calcium removal on root canal dentin after 17% EDTA, 17% EGTA, 15% EDTAC, and 1% tetracycline-HCl treatment; with or without subsequent use of 2.5% NaOCl. Extracted single-rooted human teeth were bisected longitudinally and the root halves (n=100) were isolated with nail varnish, leaving the root canal exposed. The samples were immersed in the test solutions for 1 and 5 minutes, after which the amount of calcium ion (Ca(2+)) release into the solutions was determined by flame photometry. Regardless of treatment time, all single (treatment solution only) and combined (treatment solution with subsequent NaOCl application) irrigation regimens removed significantly more Ca(2+) than control treatment (distilled water). Compared with other groups, treatment with 17% EDTA and 17% EDTA + 2.5% NaOCl resulted in the maximum amount of Ca(2+) removal from root canal dentin (p<0.05). All combined-treatment groups except 17% EGTA + 2.5% NaOCl removed significantly more Ca(2+) than their single-treatment versions (p<0.05). Within each test group, extending the treatment time to 5 minutes resulted in significantly more Ca(2+) removal (p<0.05).
本研究的目的是确定17%乙二胺四乙酸（EDTA）、17%乙二醇双乙醚二胺四乙酸（EGTA）、15%乙二胺四乙酸三钠钙（EDTAC）和1%盐酸四环素处理后根管牙本质上钙去除的程度；以及后续使用或不使用2.5%次氯酸钠（NaOCl）的情况。将拔除的单根人牙纵向平分，用指甲油隔离牙根的两半（n = 100），使根管暴露。将样本浸入测试溶液中1分钟和5分钟，之后通过火焰光度法测定溶液中钙离子（Ca(2+)）的释放量。无论处理时间如何，所有单一（仅处理溶液）和联合（处理溶液后应用NaOCl）冲洗方案去除的Ca(2+)均显著多于对照处理（蒸馏水）。与其他组相比，用17% EDTA和17% EDTA + 2.5% NaOCl处理导致从根管牙本质中去除的Ca(2+)量最大（p<0.05）。除17% EGTA + 2.5% NaOCl外，所有联合处理组去除的Ca(2+)均显著多于其单一处理组（p<0.05）。在每个测试组中，将处理时间延长至5分钟导致去除的Ca(2+)显著更多（p<0.05）。
