Lambeck Annechien J A, Crijns Anne P G, Leffers Ninke, Sluiter Wim J, ten Hoor Klaske A, Braid Mike, van der Zee Ate G J, Daemen Toos, Nijman Hans W, Kast W Martin
Department of Gynaecology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
Clin Cancer Res. 2007 Apr 15;13(8):2385-91. doi: 10.1158/1078-0432.CCR-06-1828.
To evaluate if serum cytokine levels could be used as diagnostic or prognostic markers in ovarian cancer.
A cytokine bead array was done to simultaneously analyze 14 cytokines in the sera of 187 ovarian cancer patients with complete clinicopathologic data and follow-up, 45 patients with benign ovarian tumors, and 50 healthy controls. Serum levels of the well-known serum tumor marker CA-125 were routinely measured in all patients.
Serum levels of CA-125, interleukin 6 (IL-6), IL-7, and IL-10 were elevated in ovarian cancer patients compared with patients with benign ovarian tumors. Analyzing the cytokines in combination with CA-125 showed that a combination of IL-7 and CA-125 serum levels could accurately predict 69% of the ovarian cancer patients, without falsely classifying patients with benign pelvic mass. The cytokines IL-6, IL-7, IL-8, IL-10, monocyte chemotactic protein-1 (MCP-1), and IP-10 and CA-125 were associated with disease-free and overall survival in univariate analysis. In multivariate analysis, IL-7 and IP-10 were independent predictors of overall survival, although after inclusion of the clinicopathologic parameters, only stage and residual disease remained as independent predictors of survival.
IL-7 levels were found to be strongly associated with ovarian cancer and could be used in combination with CA-125 to distinguish between malignant and benign ovarian tumors.
评估血清细胞因子水平是否可作为卵巢癌的诊断或预后标志物。
采用细胞因子微珠阵列法同时分析187例具有完整临床病理数据及随访资料的卵巢癌患者、45例卵巢良性肿瘤患者和50例健康对照者血清中的14种细胞因子。所有患者均常规检测血清中著名的肿瘤标志物CA-125水平。
与卵巢良性肿瘤患者相比，卵巢癌患者血清中CA-125、白细胞介素6（IL-6）、IL-7和IL-10水平升高。将细胞因子与CA-125联合分析显示，IL-7和CA-125血清水平联合可准确预测69%的卵巢癌患者，且不会将盆腔良性肿块患者误分类。在单因素分析中，细胞因子IL-6、IL-7、IL-8、IL-10、单核细胞趋化蛋白-1（MCP-1）、IP-10和CA-125与无病生存期和总生存期相关。在多因素分析中，IL-7和IP-10是总生存期的独立预测因素，不过在纳入临床病理参数后，只有分期和残留病灶仍是生存期的独立预测因素。
发现IL-7水平与卵巢癌密切相关，可与CA-125联合用于区分卵巢恶性肿瘤和良性肿瘤。
