印度尼西亚本土姜黄属植物中姜黄素类化合物的高效液相色谱-光电二极管阵列检测分析

HPLC-photodiode array detection analysis of curcuminoids in Curcuma species indigenous to Indonesia.

作者信息

Bos Rein, Windono Tri, Woerdenbag Herman J, Boersma Ykelien L, Koulman Albert, Kayser Oliver

机构信息

Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1,9713 AV Groningen, The Netherlands.

出版信息

Phytochem Anal. 2007 Mar-Apr;18(2):118-22. doi: 10.1002/pca.959.

DOI:10.1002/pca.959

PMID:17439012

Abstract

An optimized HPLC method with photodiode array detection was developed and applied to analyse the curcuminoids curcumin, demethoxycurcumin, and bis-demethoxycurcumin in rhizomes of Curcuma mangga Val &. v. Zijp, C. heyneana Val. & v. Zijp, C. aeruginosa Roxb. and C. soloensis Val. (Zingiberaceae), indigenous to Indonesia. The method was validated with an isocratic system, a short run time of 10 min and a baseline separation. The curcuminoid content was 0.18-0.47% for C. mangga, 0.98-3.21% for C. heyneana, 0.02-0.03% for C. aeruginosa and 0.40% for C. soloensis.

摘要

开发了一种采用光电二极管阵列检测的优化高效液相色谱法，并将其应用于分析印尼本土姜科植物芒果姜（Curcuma mangga Val &. v. Zijp）、海氏姜（C. heyneana Val. & v. Zijp）、绿姜（C. aeruginosa Roxb.）和索洛姜（C. soloensis Val.）根茎中的姜黄素类化合物姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。该方法采用等度系统进行了验证，运行时间短，为10分钟，且实现了基线分离。芒果姜的姜黄素类化合物含量为0.18 - 0.47%，海氏姜为0.98 - 3.21%，绿姜为0.02 - 0.03%，索洛姜为0.40%。