Ho Sze-Yuen, Jiang Yue, Chen Feng
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
J Agric Food Chem. 2007 May 16;55(10):3960-6. doi: 10.1021/jf0700071. Epub 2007 Apr 18.
Twenty-five isolates of Mortierella spcies were prepared, which can be used for the production of polyunsaturated fatty acids (PUFAs)-rich oil for nutritional supplements. The fatty acid contents were determined after heterotrophic fermentation. The content of total fatty acids (TFAs) in the cell dry weight of all isolates including two commercially purchased Mortierella alpina strains ranged from 207.51 to 370.11 mg/g, whereas PUFAs were the dominant fatty acid type. The highest PUFA-containing strain, M. alpina SC9, was identified and confirmed as a new strain of M. alpina through comparison analysis of the sequences of internal transcribed spacers 1 and 2 (ITS1 and ITS2) and the 5.8S rDNA region. During a 7-day fermentation, the PUFAs content of M. alpina SC9 varied between 189.83 and 240.00 mg/g, with a remarkable correlation between the oleic acid (C18:1, OA) and arachidonic acid (C20:4n-6, AA) contents and between the linoleic acid (C18:2n-6, LA) and AA contents, suggesting the PUFA content in the fungus is tightly regulated. This study provides a framework of isolation, identification, and characterization of an important PUFA-producing species, M. alpina.
制备了25株被孢霉属菌株，可用于生产富含多不饱和脂肪酸（PUFAs）的油脂以用于营养补充剂。在异养发酵后测定脂肪酸含量。包括两株市售高山被孢霉菌株在内的所有菌株的细胞干重中总脂肪酸（TFAs）含量在207.51至370.11mg/g之间，而PUFAs是主要的脂肪酸类型。通过对内部转录间隔区1和2（ITS1和ITS2）以及5.8S rDNA区域序列的比较分析，鉴定并确认含PUFAs最高的菌株高山被孢霉SC9为高山被孢霉的一个新菌株。在7天的发酵过程中，高山被孢霉SC9的PUFAs含量在189.83至240.00mg/g之间变化，油酸（C18:1，OA）与花生四烯酸（C20:4n-6，AA）含量之间以及亚油酸（C18:2n-6，LA）与AA含量之间存在显著相关性，表明该真菌中PUFAs的含量受到严格调控。本研究提供了一个对重要的PUFAs产生菌高山被孢霉进行分离、鉴定和表征的框架。
