Thakur M L, DeFulvio J, Park C H, Damjanov A, Yaghsezian H, Jungkind D, Epstein A, McAfee J G
Thomas Jefferson University, Philadelphia, PA 19107.
Int J Rad Appl Instrum B. 1991;18(6):605-12. doi: 10.1016/0883-2897(91)90030-o.
Polyclonal human IgG (IgG), antinuclear antibody (TNT-1), and human serum albumin (HSA), were labeled with 99mTc by a method recently developed in our laboratory, and administered i.v., each to a separate group of five mice, bearing inflammatory foci induced by an i.m. injection of 40 microL turpentine or 5 x 10(8) E. coli and 5 x 10(8) Entercocci. TNT-1 labeled with 125I served as a control and 67Ga-citrate as a "gold standard". At 4 or 24 h post injection, animals were imaged and sacrificed for tissue distribution studies. At 4 h in the turpentine group, the abscess-to-muscle ratios were: 67Ga, 4.8 +/- 2.1, 125I-TNT-1, 4.3 +/- 1; 99mTc-TNT-1, 3.5 +/- 1.8; 99mTc-IgG, 3.9 +/- 0.6; and 99mTc-HSA, 4.3 +/- 1. In the microorganism group, these ratios were 2.6 +/- 0.6, 3.3 +/- 0.5, 3.4 +/- 0.08, 3 +/- 1.1 and 4.1 +/- 0.6, respectively. Autoradiographic examination of infected tissues indicated that leakage of labeled proteins into interstitial space due to increased capillary permeability may be one of the major mechanisms of uptake.
采用我们实验室最近开发的方法，用99mTc标记多克隆人免疫球蛋白（IgG）、抗核抗体（TNT-1）和人血清白蛋白（HSA），然后静脉注射给单独的五组小鼠，每组小鼠分别通过肌肉注射40微升松节油或5×10⁸大肠杆菌和5×10⁸肠球菌诱导产生炎症病灶。用125I标记的TNT-1作为对照，67Ga-柠檬酸盐作为“金标准”。注射后4小时或24小时，对动物进行成像并处死以进行组织分布研究。在松节油组中，4小时时脓肿与肌肉的比值分别为：67Ga为4.8±2.1，125I-TNT-1为4.3±1；99mTc-TNT-1为3.5±1.8；99mTc-IgG为3.9±0.6；99mTc-HSA为4.3±1。在微生物组中，这些比值分别为2.6±0.6、3.3±0.5、3.4±0.08、3±1.1和4.1±0.6。对感染组织的放射自显影检查表明，由于毛细血管通透性增加导致标记蛋白渗漏到间质空间可能是摄取的主要机制之一。
