Hengge-Aronis R, Klein W, Lange R, Rimmele M, Boos W
Department of Biology, University of Konstanz, Germany.
J Bacteriol. 1991 Dec;173(24):7918-24. doi: 10.1128/jb.173.24.7918-7924.1991.
The rpoS (katF) gene of Escherichia coli encodes a putative sigma factor (sigma S) required for the expression of a variety of stationary phase-induced genes, for the development of stationary-phase stress resistance, and for long-term starvation survival (R. Lange and R. Hengge-Aronis, Mol. Microbiol. 5:49-59, 1991). Here we show that the genes otsA, otsB, treA, and osmB, previously known to be osmotically regulated, are also induced during transition into stationary phase in a sigma S-dependent manner. otsA and otsB, which encode trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase, respectively, are involved in sigma S-dependent stationary-phase thermotolerance. Neither sigma S nor trehalose, however, is required for the development of adaptive thermotolerance in growing cells, which might be controlled by sigma E.
大肠杆菌的rpoS（katF）基因编码一种假定的σ因子（σS），它是多种稳定期诱导基因表达、稳定期应激抗性发展以及长期饥饿存活所必需的（R. 兰格和R. 亨格 - 阿罗尼斯，《分子微生物学》5:49 - 59，1991）。在此我们表明，先前已知受渗透压调节的otsA、otsB、treA和osmB基因，在进入稳定期的转变过程中也以σS依赖的方式被诱导。分别编码海藻糖 - 6 - 磷酸合酶和海藻糖 - 6 - 磷酸磷酸酶的otsA和otsB，参与了σS依赖的稳定期耐热性。然而，生长中的细胞适应性耐热性的发展既不需要σS也不需要海藻糖，这可能由σE控制。
