Zhou Chao, Zhou Yan-fei, Fan Tian-yuan
Department of Pharmaceutics, Peking University School of Pharmaceutical Sciences, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2007 Apr 18;39(2):182-5.
To develop and characterize the hydrogel microspheres for embolization.
N-[tris (hydroxymethyl) methyl] acrylamide-gelatin microspheres (TGMs) were prepared by an inverse suspension polymerization approach. Effects of materials on size, water absorption rate and elasticity of the microspheres were investigated. The materials which were included consisted of gelatin in the range of 10.0-100.0 g/L, N-[tris (hydroxymethyl)methyl]acrylamide in the range of 33.3-200 g/L, cross-linking agent N,N'-methylene-bis-acrylamide in the range of 3.3-10.0 g/L, surfactant Span 80 in the range of 0.5-1.8 g/L, and initiator ammonium persulfate in the range of 1.0-5.0 g/L. The appearance of TGMs was observed under microscope. TGMs were analyzed by infrared spectrum (IR).
The TGMs were round with smooth surface in view of photograph of microscope. The average diameter of TGMs was increased with the increase of gelatin, monomer or cross-linking agent concentrations but decreased with the increase of surfactant or initiator Concentration. The water adsorption rate of the microspheres was decreased with the increase of gelatin or cross-linking agent concentration but not affected by surfactant concentration. The elasticity of TGMs was increased with the increase of monomer or cross-linking agent concentration, decreased with the increase of gelatin concentration, but not affected by surfactant or initiator concentration. All factors above considered, the final prepared TGMs consisted of 10 g/L gelatin, 100.0 g/L monomer, 6.7 g/L cross-linking agent, 0.9 g/L surfactant, and 3.0 g/L initiator. The average diameter of TGMs obtained was about 700.0 microm. The water adsorption rate and the elasticity in accordance with the maximum diameter of the microspheres passed through a microcatheter of TGMs were 12.4 (g/g), and 1 600.0 microm, respectively. The results of IR spectra confirmed the polymerization of monomer, resulting in Nj[tris(hydroxymethyl)methyl]acrylamidegelatin microspheres.
The developed TGMs seemed to be suitable for clinical embolization according to the surface, average diameter, elastic and hydrophilic property of TGMs.
研发用于栓塞的水凝胶微球并对其进行特性表征。
采用反相悬浮聚合法制备N-[三（羟甲基）甲基]丙烯酰胺-明胶微球（TGMs）。研究了材料对微球尺寸、吸水率和弹性的影响。所包含的材料有10.0 - 100.0 g/L范围内的明胶、33.3 - 200 g/L范围内的N-[三（羟甲基）甲基]丙烯酰胺、3.3 - 10.0 g/L范围内的交联剂N,N'-亚甲基双丙烯酰胺、0.5 - 1.8 g/L范围内的表面活性剂Span 80以及1.0 - 5.0 g/L范围内的引发剂过硫酸铵。在显微镜下观察TGMs的外观。通过红外光谱（IR）对TGMs进行分析。
从显微镜照片来看，TGMs呈圆形且表面光滑。TGMs的平均直径随明胶、单体或交联剂浓度的增加而增大，但随表面活性剂或引发剂浓度的增加而减小。微球的吸水率随明胶或交联剂浓度的增加而降低，但不受表面活性剂浓度的影响。TGMs的弹性随单体或交联剂浓度的增加而增大，随明胶浓度的增加而减小，但不受表面活性剂或引发剂浓度的影响。综合考虑上述所有因素，最终制备的TGMs由10 g/L明胶、100.0 g/L单体、6.7 g/L交联剂、0.9 g/L表面活性剂和3.0 g/L引发剂组成。所获得的TGMs的平均直径约为700.0微米。TGMs通过微导管的微球最大直径对应的吸水率和弹性分别为12.4（g/g）和1600.0微米。红外光谱结果证实了单体的聚合，形成了N-[三（羟甲基）甲基]丙烯酰胺明胶微球。
根据TGMs的表面、平均直径、弹性和亲水性能，所研发的TGMs似乎适用于临床栓塞。
