Wu Ya-nan, Cui Dai-chao, Liu Ying-ying, Fan Tian-yuan
Department of Pharmaceutics, Peking University School of Pharmaceutical Sciences, Beijing, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2012 Jun 18;44(3):464-8.
To optimize the preparation of gelatin microspheres for embolization and to evaluate the physicochemical properties of the optimized microspheres.
The gelatin microspheres were prepared using emulsification crosslink method. The response surface methodology (RSM) was used in order to achieve gelatin microspheres with satisfactory degradable time and elasticity. The response values considered in this study were degradable time and elasticity, and the factors were the concentration of formaldehyde solution and the time of cross-linking reaction. The optimized microspheres were achieved by RSM. The properties of the optimized microspheres were investigated, including degradable time, elasticity, particle size, ratio of water absorption and the swelling ratio.
The elasticity of the optimized microspheres was appropriate. The degradable time of the optimized microspheres was 2-3 weeks. The average diameter for dried gelatin microsphere was 377.6 μm, and for wet gelatin microsphere was 535.6 μm. The gelatin microsphere achieved the rate of water absorption balance at 20 min, and the average swelling ratio of gelatin microsphere was 41.9%.
The gelatin microspheres optimized by RSM seemed to be suitable for clinical embolization according to the physicochemical properties.
优化用于栓塞的明胶微球的制备方法，并评估优化后的微球的理化性质。
采用乳化交联法制备明胶微球。为了获得具有令人满意的降解时间和弹性的明胶微球，使用了响应面法（RSM）。本研究中考虑的响应值为降解时间和弹性，因素为甲醛溶液浓度和交联反应时间。通过响应面法获得优化后的微球。对优化后的微球的性质进行了研究，包括降解时间、弹性、粒径、吸水率和溶胀率。
优化后的微球弹性适宜。优化后的微球的降解时间为2至3周。干燥明胶微球的平均直径为377.6μm，湿明胶微球的平均直径为535.6μm。明胶微球在20分钟时达到吸水平衡率，明胶微球的平均溶胀率为41.9%。
根据理化性质，经响应面法优化的明胶微球似乎适用于临床栓塞。
